Abstract
Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue-derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31-CD34+CD45-CD90-CD105 -CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31 -CD34+CD45-CD90-CD105 -CD146- population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood-derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose-derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue.
| Original language | English |
|---|---|
| Pages (from-to) | 440-447 |
| Number of pages | 8 |
| Journal | Cell Biochemistry and Function |
| Volume | 27 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Oct 1 2009 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biochemistry
- Clinical Biochemistry
- Cell Biology
Cite this
Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues. / Toyoda, Mito; Matsubara, Yoshinori; Lin, Konghua; Sugimachi, Keizou; Furue, Masutaka.
In: Cell Biochemistry and Function, Vol. 27, No. 7, 01.10.2009, p. 440-447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues
AU - Toyoda, Mito
AU - Matsubara, Yoshinori
AU - Lin, Konghua
AU - Sugimachi, Keizou
AU - Furue, Masutaka
PY - 2009/10/1
Y1 - 2009/10/1
N2 - Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue-derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31-CD34+CD45-CD90-CD105 -CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31 -CD34+CD45-CD90-CD105 -CD146- population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood-derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose-derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue.
AB - Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue-derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31-CD34+CD45-CD90-CD105 -CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31 -CD34+CD45-CD90-CD105 -CD146- population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood-derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose-derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue.
UR - http://www.scopus.com/inward/record.url?scp=70350578622&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350578622&partnerID=8YFLogxK
U2 - 10.1002/cbf.1591
DO - 10.1002/cbf.1591
M3 - Article
C2 - 19691084
AN - SCOPUS:70350578622
VL - 27
SP - 440
EP - 447
JO - Cell Biochemistry and Function
JF - Cell Biochemistry and Function
SN - 0263-6484
IS - 7
ER -