Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues

Mito Toyoda, Yoshinori Matsubara, Konghua Lin, Keizou Sugimachi, Masutaka Furue

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue-derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31-CD34+CD45-CD90-CD105 -CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31 -CD34+CD45-CD90-CD105 -CD146- population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood-derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose-derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue.

Original languageEnglish
Pages (from-to)440-447
Number of pages8
JournalCell Biochemistry and Function
Volume27
Issue number7
DOIs
Publication statusPublished - Oct 1 2009

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Subcutaneous Fat
Adipose Tissue
Tissue
Stem Cells
Adipocytes
Stem cells
Population
Fats
Pericytes
Regenerative Medicine
Subcutaneous Tissue
Vascular Smooth Muscle
Cells
Smooth Muscle Myocytes
Blood Cells
Flow Cytometry
Flow cytometry
Phenotype
Muscle
Blood

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues. / Toyoda, Mito; Matsubara, Yoshinori; Lin, Konghua; Sugimachi, Keizou; Furue, Masutaka.

In: Cell Biochemistry and Function, Vol. 27, No. 7, 01.10.2009, p. 440-447.

Research output: Contribution to journalArticle

Toyoda, Mito ; Matsubara, Yoshinori ; Lin, Konghua ; Sugimachi, Keizou ; Furue, Masutaka. / Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues. In: Cell Biochemistry and Function. 2009 ; Vol. 27, No. 7. pp. 440-447.
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