Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination

Takako Takanami, Shusei Sato, Takeshi Ishihara, Isao Katsura, Hideyuki Takahashi, Atsushi Higashitani

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.

Original languageEnglish
Pages (from-to)373-377
Number of pages5
JournalDNA Research
Volume5
Issue number6
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

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Caenorhabditis elegans
Genetic Recombination
Genes
Meiotic Prophase I
Oogenesis
Chromosomes, Human, Pair 1
RNA Interference
Eukaryota
Eggs
Oocytes
Amino Acid Sequence
Genome
Databases
Amino Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination. / Takanami, Takako; Sato, Shusei; Ishihara, Takeshi; Katsura, Isao; Takahashi, Hideyuki; Higashitani, Atsushi.

In: DNA Research, Vol. 5, No. 6, 01.01.1998, p. 373-377.

Research output: Contribution to journalArticle

Takanami, Takako ; Sato, Shusei ; Ishihara, Takeshi ; Katsura, Isao ; Takahashi, Hideyuki ; Higashitani, Atsushi. / Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination. In: DNA Research. 1998 ; Vol. 5, No. 6. pp. 373-377.
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