Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus

Minoru Takeshita, Yuka Matsuo, Takaya Yoshikawa, Masashi Suzuki, Naruto Furuya, Kenichi Tsuchiya, Yoichi Takanami

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A defective (D) RNA 3 naturally generated from the Y strain of cucumber mosaic virus (CMV-Y) was characterized using a biologically active cDNA clone. Sequencing of the clone revealed that the D RNA 3, named D RNA 3Yα, was derived from CMV-Y RNA 3 and contained a 10 nt deletion in the 5′ untranslated region and a 162 nt deletion within the 3a open reading frame. Co-inoculation of D RNA 3Yα with CMV-Y derived from in vitro transcripts facilitated propagation of CMV-Y containing D RNA 3Yα in Nicotiana benthamiana or Nicotiana tabacum plants. CMV-Y, however, did not produce deletion mutants upon serial mechanical passages in the plants.

Original languageEnglish
Pages (from-to)579-583
Number of pages5
JournalArchives of Virology
Volume153
Issue number3
DOIs
Publication statusPublished - Mar 2008

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Cucumovirus
RNA
Tobacco
Clone Cells
Serial Passage
5' Untranslated Regions
Open Reading Frames
Complementary DNA
DNA-like RNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Takeshita, M., Matsuo, Y., Yoshikawa, T., Suzuki, M., Furuya, N., Tsuchiya, K., & Takanami, Y. (2008). Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus. Archives of Virology, 153(3), 579-583. https://doi.org/10.1007/s00705-007-0026-1

Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus. / Takeshita, Minoru; Matsuo, Yuka; Yoshikawa, Takaya; Suzuki, Masashi; Furuya, Naruto; Tsuchiya, Kenichi; Takanami, Yoichi.

In: Archives of Virology, Vol. 153, No. 3, 03.2008, p. 579-583.

Research output: Contribution to journalArticle

Takeshita, M, Matsuo, Y, Yoshikawa, T, Suzuki, M, Furuya, N, Tsuchiya, K & Takanami, Y 2008, 'Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus', Archives of Virology, vol. 153, no. 3, pp. 579-583. https://doi.org/10.1007/s00705-007-0026-1
Takeshita, Minoru ; Matsuo, Yuka ; Yoshikawa, Takaya ; Suzuki, Masashi ; Furuya, Naruto ; Tsuchiya, Kenichi ; Takanami, Yoichi. / Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus. In: Archives of Virology. 2008 ; Vol. 153, No. 3. pp. 579-583.
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