Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus

Minoru Takeshita, Yuka Matsuo, Takaya Yoshikawa, Masashi Suzuki, Naruto Furuya, Kenichi Tsuchiya, Yoichi Takanami

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A defective (D) RNA 3 naturally generated from the Y strain of cucumber mosaic virus (CMV-Y) was characterized using a biologically active cDNA clone. Sequencing of the clone revealed that the D RNA 3, named D RNA 3Yα, was derived from CMV-Y RNA 3 and contained a 10 nt deletion in the 5′ untranslated region and a 162 nt deletion within the 3a open reading frame. Co-inoculation of D RNA 3Yα with CMV-Y derived from in vitro transcripts facilitated propagation of CMV-Y containing D RNA 3Yα in Nicotiana benthamiana or Nicotiana tabacum plants. CMV-Y, however, did not produce deletion mutants upon serial mechanical passages in the plants.

Original languageEnglish
Pages (from-to)579-583
Number of pages5
JournalArchives of Virology
Volume153
Issue number3
DOIs
Publication statusPublished - Mar 2008

All Science Journal Classification (ASJC) codes

  • Genetics
  • Applied Microbiology and Biotechnology

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