Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1

Yuji Aso, Toshihiro Sashihara, Jun Ichi Nagao, Youhei Kanemasa, Hanako Koga, Taku Hashimoto, Toshimasa Higuchi, Asaho Adachi, Harumi Nomiyama, Ayaaki Ishizaki, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

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Abstract

We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type (antibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit.

Original languageEnglish
Pages (from-to)1663-1671
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume68
Issue number8
DOIs
Publication statusPublished - Aug 1 2004

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Bacteriocins
Biosynthesis
Multigene Family
Staphylococcus
Immunity
Genes
DNA sequences
Osmotic Pressure
Post Translational Protein Processing
Operon
Open Reading Frames
Plasmids
nukacin ISK-1
Anti-Bacterial Agents
Membranes
Networks (circuits)

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1. / Aso, Yuji; Sashihara, Toshihiro; Nagao, Jun Ichi; Kanemasa, Youhei; Koga, Hanako; Hashimoto, Taku; Higuchi, Toshimasa; Adachi, Asaho; Nomiyama, Harumi; Ishizaki, Ayaaki; Nakayama, Jiro; Sonomoto, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 68, No. 8, 01.08.2004, p. 1663-1671.

Research output: Contribution to journalArticle

Aso, Y, Sashihara, T, Nagao, JI, Kanemasa, Y, Koga, H, Hashimoto, T, Higuchi, T, Adachi, A, Nomiyama, H, Ishizaki, A, Nakayama, J & Sonomoto, K 2004, 'Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1', Bioscience, Biotechnology and Biochemistry, vol. 68, no. 8, pp. 1663-1671. https://doi.org/10.1271/bbb.68.1663
Aso, Yuji ; Sashihara, Toshihiro ; Nagao, Jun Ichi ; Kanemasa, Youhei ; Koga, Hanako ; Hashimoto, Taku ; Higuchi, Toshimasa ; Adachi, Asaho ; Nomiyama, Harumi ; Ishizaki, Ayaaki ; Nakayama, Jiro ; Sonomoto, Kenji. / Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1. In: Bioscience, Biotechnology and Biochemistry. 2004 ; Vol. 68, No. 8. pp. 1663-1671.
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