Characterization of a nonsense mutation in FAD7, the gene which encodes ω-3 desaturase in Arabidopsis thaliana

Junko Kusumi; Koh Iba

Characterization of a nonsense mutation in FAD7, the gene which encodes ω-3 desaturase in Arabidopsis thaliana

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Abstract

The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.

Original language	English
Pages (from-to)	87-91
Number of pages	5
Journal	Journal of Plant Research
Volume	111
Issue number	1
Publication status	Published - Mar 1998

All Science Journal Classification (ASJC) codes

Plant Science

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title = "Characterization of a nonsense mutation in FAD7, the gene which encodes ω-3 desaturase in Arabidopsis thaliana",

abstract = "The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.",

author = "Junko Kusumi and Koh Iba",

year = "1998",

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language = "English",

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journal = "Journal of Plant Research",

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AU - Kusumi, Junko

AU - Iba, Koh

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N2 - The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.

AB - The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.

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M3 - Article

SN - 0918-9440

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Characterization of a nonsense mutation in FAD7, the gene which encodes ω-3 desaturase in Arabidopsis thaliana

Abstract

All Science Journal Classification (ASJC) codes

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