Characterization of a nonsense mutation in FAD7, the gene which encodes ω-3 desaturase in Arabidopsis thaliana

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Abstract

The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.

Original languageEnglish
Pages (from-to)87-91
Number of pages5
JournalJournal of Plant Research
Volume111
Issue number1101
Publication statusPublished - Mar 1998

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nonsense mutation
Arabidopsis thaliana
mutants
genes
plastids
Arabidopsis
genetically modified organisms
genomics

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

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title = "Characterization of a nonsense mutation in FAD7, the gene which encodes ω-3 desaturase in Arabidopsis thaliana",
abstract = "The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.",
author = "Junko Kusumi and Koh Iba",
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AU - Iba, Koh

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N2 - The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.

AB - The Arabidopsis fad7-1 mutant is deficient in the activity of plastid ω-3 desaturase as a direct consequence of being devoid of FAD7 mRNA. Comparison of the genomic sequence from the fad7-1 mutant line (JB101) with the wild-type sequence revealed that the only single-base pair transition occurred within the FAD7 gene-coding region. Transgenic study and northern analysis both indicated that the absence of FAD7 mRNA in the fad7-1 mutant was not caused by any disability regarding transcriptional regulation, but by the destabilization of the mRNA.

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