Characterization of a novel 550-kDa protein in skeletal muscle of chick embryo

Akihito Hattori, Jun ichi Wakamatsu, Tatsuo Ishii, Kyoko Kuwahara, Ryuichi Tatsumi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Some characteristics of a novel 550-kDa protein which is abundant in skeletal muscle tissues at an early stage of the chick embryo, and localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils, was investigated. A cosedimentation experiment and solid phase immunoabsorbent assay showed that the 550-kDa protein binds directly to F-actin. Therefore, it is concluded that the 550-kDa protein is a novel actin-binding protein. The 550-kDa protein was also interacted with α-actinin, laminin, fibronectin and Type IV collagen. Reactions with several kinds of lectin revealed that the 550-kDa protein is a glycoprotein containing oligosaccharides. Electron microscopic observation of negatively stained 550-kDa protein showed that native 550-kDa protein molecules are particles with an average diameter of 26.5 nm, but those particles treated with ethanol/ether are filamentous structures. These results suggest that the 550-kDa protein in the cytoplasm of unorganized skeletal muscle tissues exists as lipid-protein complex. Consequently, the 550-kDa protein may play an important role in the binding of myofibrils to the basal lamina by interaction with F-actin, α-actinin, laminin, fibronectin or Type IV collagen.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalBBA - General Subjects
Volume1245
Issue number2
DOIs
Publication statusPublished - Oct 19 1995
Externally publishedYes

Fingerprint

Chick Embryo
Muscle
Skeletal Muscle
Proteins
Actinin
Collagen Type IV
Myofibrils
Laminin
Fibronectins
Muscles
Actins
Tissue
Microfilament Proteins
Oligosaccharides
Lectins
Basement Membrane
Ether
Assays
Glycoproteins
Cytoplasm

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Characterization of a novel 550-kDa protein in skeletal muscle of chick embryo. / Hattori, Akihito; Wakamatsu, Jun ichi; Ishii, Tatsuo; Kuwahara, Kyoko; Tatsumi, Ryuichi.

In: BBA - General Subjects, Vol. 1245, No. 2, 19.10.1995, p. 201-206.

Research output: Contribution to journalArticle

Hattori, Akihito ; Wakamatsu, Jun ichi ; Ishii, Tatsuo ; Kuwahara, Kyoko ; Tatsumi, Ryuichi. / Characterization of a novel 550-kDa protein in skeletal muscle of chick embryo. In: BBA - General Subjects. 1995 ; Vol. 1245, No. 2. pp. 201-206.
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