Characterization of a novel superoxide dismutase in Nilaparvata lugens

Kohji Yamamoto, Misuzu Yamaguchi

Research output: Contribution to journalArticlepeer-review

Abstract

The brown planthopper (Nilaparvata lugens) is a major agricultural pest of rice crops. Analysis of the enzymes produced by N. lugens is important to develop pest-control methods. Superoxide dismutase (SOD) is a detoxification enzyme that catalyzes the conversion of superoxide anions (reactive oxygen species) into oxygen and hydrogen peroxide. As there have been no reports on SOD in N. lugens, in this study, we characterized a new SOD in the brown planthopper, nlSOD1. Amino acid sequence and phylogenetic analyses revealed that nlSOD1 is a member of the Cu/Zn-SOD family. Recombinant nlSOD1, when overexpressed in Escherichia coli, catalyzes the dismutation of superoxide radicals into molecular O2 and H2O2. Exposure to various insecticides induced nlSOD1 messenger RNA expression. These results indicate that nlSOD1 may contribute to the insecticide resistance of N. lugens. The findings of this study may assist in the development of novel methods to control the population of N. lugens.

Original languageEnglish
Article numbere21862
JournalArchives of insect biochemistry and physiology
Volume109
Issue number2
DOIs
Publication statusPublished - Feb 2022

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Insect Science

Fingerprint

Dive into the research topics of 'Characterization of a novel superoxide dismutase in Nilaparvata lugens'. Together they form a unique fingerprint.

Cite this