Characterization of a novel thogotovirus isolated from Amblyomma testudinarium ticks in Ehime, Japan: A significant phylogenetic relationship to Bourbon virus

Hiroko Ejiri, Chang Kweng Lim, Haruhiko Isawa, Ryosuke Fujita, Katsunori Murota, Tomomi Sato, Daisuke Kobayashi, Miki Kan, Masashi Hattori, Toshiya Kimura, Yukie Yamaguchi, Mutsuyo Takayama-Ito, Madoka Horiya, Guillermo Posadas-Herrera, Shohei Minami, Ryusei Kuwata, Hiroshi Shimoda, Ken Maeda, Yukie Katayama, Tetsuya MizutaniMasayuki Saijo, Koki Kaku, Hiroto Shinomiya, Kyoko Sawabe

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The genus Thogotovirus, as represented by Thogoto virus and Dhori virus, comprises a group of arthropod-borne viruses, most members of which are transmitted by ticks. Here we report the genetic and biological characterization of a new thogotovirus, designated Oz virus (OZV), isolated from the hard tick Amblyomma testudinarium in Ehime, Japan. OZV efficiently replicated and induced a cytopathic effect in Vero cells, from which enveloped pleomorphic virus particles were formed by budding. OZV could also replicate in BHK-21 and DH82 cells and caused high mortality in suckling mice after intracerebral inoculation. Phylogenetic analyses of six viral proteins indicated that OZV is clustered with Dhori and related viruses, and is most closely related in glycoprotein (GP) and matrix protein (M) sequences to Bourbon virus, a human-pathogenic thogotovirus discovered recently in the United States. Our findings emphasize the need for understanding the geographic distribution and ecology of OZV and related viruses and for reevaluation of the medical and public health importance of thogotoviruses.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalVirus Research
Volume249
DOIs
Publication statusPublished - Apr 2 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Virology
  • Infectious Diseases

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