Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity

Naotaka Tanaka, Mami Konomi, Masako Osumi, Kaoru Takegawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In fission yeast, Schizosaccharomyces pombe, the carbohydrate components of the cell wall consist of galactomannan, unlike in Saccharomyces cerevisiae. We previously found that the disruption of gms1+, a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in Sz. pombe. The Δgms1 strain is therefore useful for the analysis of physiological properties of galactose residues in Sz. pombe. The deletion strain of gms1+ was viable; however, it showed an aberrant cell morphology and increased sensitivities to digestion with β-glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Δgms1 strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Δgms1 cells, while other phenotypes were weakly suppressed. The Δgms1 (h90) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non-sexual flocculation but also for sexual conjugation in Sz. pombe.

Original languageEnglish
Pages (from-to)903-914
Number of pages12
JournalYeast
Volume18
Issue number10
DOIs
Publication statusPublished - Aug 6 2001
Externally publishedYes

Fingerprint

Uridine Diphosphate Galactose
Schizosaccharomyces
Yeast
Cell Wall
Hygromycin B
Cells
Flocculation
Defects
Gene encoding
Vanadates
Carbohydrates
Starvation
Galactose
Saccharomyces cerevisiae
Digestion
Sodium
Electrons
Scanning
Phenotype
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity. / Tanaka, Naotaka; Konomi, Mami; Osumi, Masako; Takegawa, Kaoru.

In: Yeast, Vol. 18, No. 10, 06.08.2001, p. 903-914.

Research output: Contribution to journalArticle

Tanaka, Naotaka ; Konomi, Mami ; Osumi, Masako ; Takegawa, Kaoru. / Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity. In: Yeast. 2001 ; Vol. 18, No. 10. pp. 903-914.
@article{3483bbe633f64da5bddab199a81273c7,
title = "Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity",
abstract = "In fission yeast, Schizosaccharomyces pombe, the carbohydrate components of the cell wall consist of galactomannan, unlike in Saccharomyces cerevisiae. We previously found that the disruption of gms1+, a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in Sz. pombe. The Δgms1 strain is therefore useful for the analysis of physiological properties of galactose residues in Sz. pombe. The deletion strain of gms1+ was viable; however, it showed an aberrant cell morphology and increased sensitivities to digestion with β-glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Δgms1 strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Δgms1 cells, while other phenotypes were weakly suppressed. The Δgms1 (h90) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non-sexual flocculation but also for sexual conjugation in Sz. pombe.",
author = "Naotaka Tanaka and Mami Konomi and Masako Osumi and Kaoru Takegawa",
year = "2001",
month = "8",
day = "6",
doi = "10.1002/yea.740",
language = "English",
volume = "18",
pages = "903--914",
journal = "Yeast",
issn = "0749-503X",
publisher = "John Wiley and Sons Ltd",
number = "10",

}

TY - JOUR

T1 - Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity

AU - Tanaka, Naotaka

AU - Konomi, Mami

AU - Osumi, Masako

AU - Takegawa, Kaoru

PY - 2001/8/6

Y1 - 2001/8/6

N2 - In fission yeast, Schizosaccharomyces pombe, the carbohydrate components of the cell wall consist of galactomannan, unlike in Saccharomyces cerevisiae. We previously found that the disruption of gms1+, a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in Sz. pombe. The Δgms1 strain is therefore useful for the analysis of physiological properties of galactose residues in Sz. pombe. The deletion strain of gms1+ was viable; however, it showed an aberrant cell morphology and increased sensitivities to digestion with β-glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Δgms1 strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Δgms1 cells, while other phenotypes were weakly suppressed. The Δgms1 (h90) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non-sexual flocculation but also for sexual conjugation in Sz. pombe.

AB - In fission yeast, Schizosaccharomyces pombe, the carbohydrate components of the cell wall consist of galactomannan, unlike in Saccharomyces cerevisiae. We previously found that the disruption of gms1+, a gene encoding the UDP-galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in Sz. pombe. The Δgms1 strain is therefore useful for the analysis of physiological properties of galactose residues in Sz. pombe. The deletion strain of gms1+ was viable; however, it showed an aberrant cell morphology and increased sensitivities to digestion with β-glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Δgms1 strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Δgms1 cells, while other phenotypes were weakly suppressed. The Δgms1 (h90) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non-sexual flocculation but also for sexual conjugation in Sz. pombe.

UR - http://www.scopus.com/inward/record.url?scp=0034911880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034911880&partnerID=8YFLogxK

U2 - 10.1002/yea.740

DO - 10.1002/yea.740

M3 - Article

VL - 18

SP - 903

EP - 914

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 10

ER -