Characterization of a testicular 17α,20β-dihydroxy-4-pregnen-3-one (a spermiation-inducing steroid in fish) receptor from a teleost, Japanese eel (Anguilla japonica)

Takashi Todo, Toshitaka Ikeuchi, Tohru Kobayashi, Hiroko Kajiura-Kobayashi, Kunimasa Suzuki, Michiyasu Yoshikuni, Kohei Yamauchi, Yoshitaka Nagahama

Research output: Contribution to journalArticle

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Abstract

A cDNA encoding a nuclear 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP, spermiation-inducing hormone in fish) receptor (DPR) was, for the first time, isolated from an eel testis cDNA library. The amino acid sequence of DPR shows high homology with those of human and chicken progesterone receptors. The affinity of the bacterial recombinant DPR ligand binding domain protein for 17α,20β-DP is higher than that of progesterone. In transfection experiments using COS7 cells, the DPR showed progestin-dependent activation of transcription. 17α,20β-DP was the most effective activator of transcription. These results indicate that the cDNA encodes a functional eel DPR, and show that 17α,20β-DP has a nuclear receptor-mediated action in eel testes. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)12-17
Number of pages6
JournalFEBS Letters
Volume465
Issue number1
DOIs
Publication statusPublished - Jan 7 2000

Fingerprint

Anguilla
Eels
Transcription
Fish
Fishes
Complementary DNA
Steroids
Testis
Progestins
Progesterone Receptors
Cytoplasmic and Nuclear Receptors
Gene Library
Progesterone
Chemical activation
Hormones
Ligands
Amino Acids
Transcriptional Activation
Transfection
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Characterization of a testicular 17α,20β-dihydroxy-4-pregnen-3-one (a spermiation-inducing steroid in fish) receptor from a teleost, Japanese eel (Anguilla japonica). / Todo, Takashi; Ikeuchi, Toshitaka; Kobayashi, Tohru; Kajiura-Kobayashi, Hiroko; Suzuki, Kunimasa; Yoshikuni, Michiyasu; Yamauchi, Kohei; Nagahama, Yoshitaka.

In: FEBS Letters, Vol. 465, No. 1, 07.01.2000, p. 12-17.

Research output: Contribution to journalArticle

Todo, Takashi ; Ikeuchi, Toshitaka ; Kobayashi, Tohru ; Kajiura-Kobayashi, Hiroko ; Suzuki, Kunimasa ; Yoshikuni, Michiyasu ; Yamauchi, Kohei ; Nagahama, Yoshitaka. / Characterization of a testicular 17α,20β-dihydroxy-4-pregnen-3-one (a spermiation-inducing steroid in fish) receptor from a teleost, Japanese eel (Anguilla japonica). In: FEBS Letters. 2000 ; Vol. 465, No. 1. pp. 12-17.
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