Characterization of an intermediate filament protein from the platyhelminth, Dugesia japonica

Akiko Yamamoto, Ken Ichiro Matsunaga, Toyoaki Anai, Hitoshi Kawano, Toshihisa Ueda, Toshihiko Matsumoto, Shoji Ando

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Intermediate filaments (IFs) are major constituents of the cytoskeletal systems in animal cells. Objective: To gain insights into the structure-function relationship of invertebrate cytoplasmic IF proteins, we characterized an IF protein from the platyhelminth, Dugesia japonica, termed Dif-1. Methods: cDNA cloning, in situ hybridization, immunohistochemical analysis, and IF assembly experiments in vitro using recombinant Dif-1, were performed for protein characterization. Results: The structure deduced from the cDNA sequence showed that Djf-1 comprises 568 amino acids and has a tripartite domain structure (N-terminal head, central rod, and C-terminal tail) that is characteristic of IF proteins. Similar to nuclear IF lamins, Djf-1 contains an extra 42 residues in the coil 1b subdomain of the rod domain that is absent from vertebrate cytoplasmic IF proteins and a nuclear lamin-homology segment of approximately 105 residues in the tail domain; however, it contains no nuclear localization signal. In situ hybridization analysis showed that Djf-1 mRNA is specifically expressed in cells located within the marginal region encircling the worm body. Immunohistochemical analysis showed that Djf-1 protein forms cytoplasmic IFs located close to the microvilli of the cells. In vitro IF assembly experiments using recombinant proteins showed that Djf-1 alone polymerizes into IFs. Deletion of the extra 42 residues in the coil 1b subdomain resulted in the failure of IF formation. Conclusion: Together with data from other histological studies, our results suggest that Djf-1 is expressed specifically in anchor cells within the glandular adhesive organs of the worm and that Djf-1 IFs may play a role in protecting the cells from mechanical stress.

Original languageEnglish
Pages (from-to)432-446
Number of pages15
JournalProtein and Peptide Letters
Volume27
Issue number5
DOIs
Publication statusPublished - 2020
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

Fingerprint

Dive into the research topics of 'Characterization of an intermediate filament protein from the platyhelminth, Dugesia japonica'. Together they form a unique fingerprint.

Cite this