TY - JOUR
T1 - Characterization of Arabidopsis mutants that are associated with altered C18 unsaturated fatty acid metabolism
AU - Horiguchi, Gorou
AU - Kodama, Hiroaki
AU - Iba, Koh
N1 - Funding Information:
G. Horiguchi is a recipient of a scholarship from the Japanese Society for Promotion of Science. We thank the Arabidopsis Biological Resource Center for mutant seeds and Cereon Genomics for accessing polymorphism information. Part of this study was supported by grant RFTF96L00602 from the Japanese Society for Promotion of Science.
PY - 2001
Y1 - 2001
N2 - Polyunsaturated fatty acids are a major component of glycerolipids in plant cellular membranes. We have isolated four novel fatty acid mutants of Arabidopsis thaliana named regulator of fatty acid composition (rfc) 1 through 4. The rfcl, rfc2 and rfc3 mutants had an increased oleic acid (18:1) level at the expense of α-linolenic acid (18:3) level among total fatty acids especially in root tissues. In these mutants, the transcripts of FAD2 and FAD3 gene for ER Δ12 and ω-3 fatty acid desaturases that are involved in the desaturation of 18:1 and linoleic acid (18:2), respectively, were accumulated to the wild-type levels. In the rfc4 mutant, over-accumulated 18:3 with a corresponding increase in the FAD3 mRNA over wild-type levels was observed. These results suggest that C18 unsaturated fatty acid metabolism in extra-plastid membranes is controlled by multiple loci in addition to the structural genes for fatty acid desaturases.
AB - Polyunsaturated fatty acids are a major component of glycerolipids in plant cellular membranes. We have isolated four novel fatty acid mutants of Arabidopsis thaliana named regulator of fatty acid composition (rfc) 1 through 4. The rfcl, rfc2 and rfc3 mutants had an increased oleic acid (18:1) level at the expense of α-linolenic acid (18:3) level among total fatty acids especially in root tissues. In these mutants, the transcripts of FAD2 and FAD3 gene for ER Δ12 and ω-3 fatty acid desaturases that are involved in the desaturation of 18:1 and linoleic acid (18:2), respectively, were accumulated to the wild-type levels. In the rfc4 mutant, over-accumulated 18:3 with a corresponding increase in the FAD3 mRNA over wild-type levels was observed. These results suggest that C18 unsaturated fatty acid metabolism in extra-plastid membranes is controlled by multiple loci in addition to the structural genes for fatty acid desaturases.
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U2 - 10.1016/S0168-9452(01)00523-4
DO - 10.1016/S0168-9452(01)00523-4
M3 - Article
AN - SCOPUS:0035661422
SN - 0168-9452
VL - 161
SP - 1117
EP - 1123
JO - Plant Science
JF - Plant Science
IS - 6
ER -