Characterization of B16 melanoma-specific cytotoxic T lymphocytes

Mamoru Harada, Koji Tamada, Koichiro Abe, Tieli Li, Yasuhiro Onoe, Hitoshi Tada, Katsunori Tatsugami, Takashi Ando, Genki Kimura, Kikuo Nomoto

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16 Citations (Scopus)

Abstract

A B16 melanoma-specific CD8+ T cell line (AB1) was established from the spleen cells of C57BL/6 mice cured of B16 melanoma with interleukin (IL)-12 treatment. The ABl line exclusively used T cell receptor V(β11). The AB1 cells exhibited a cytolytic activity against both syngeneic B16 melanoma and allogeneic P815 mastocytoma, whereas a cold inhibition assay revealed specificity of the AB1 cells against B16 melanoma. Their lostability to kill a class I loss variant of B16 melanoma was restored by the transfection of H- 2K(b) gene. In addition, their interferon (IFN)-γ production was significantly suppressed by the addition of anti-H-2K(b) monoclonal antibody, and RT-PCR analysis showed that the AB1 line expressed the mRNA encoding IFN- γ, but not IL-4 or IL-10. The experiment using synthetic peptides of tyrosinase-related protein-2 (TRP-2) revealed that the AB1 cells could recognize TRP-2181-188 peptide. Moreover, the AB1 cells showed an in vivo antitumor effect against established pulmonary metastases of B16 melanoma. Overall, these results indicate that the Tcl-type V(β11) + AB1 cells exert an antitumor activity against syngeneic B16 melanoma through recognition of TRP-2181-188 peptide in an H-2K(b)-restricted manner.

Original languageEnglish
Pages (from-to)198-204
Number of pages7
JournalCancer Immunology Immunotherapy
Volume47
Issue number4
DOIs
Publication statusPublished - Dec 1 1998

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

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  • Cite this

    Harada, M., Tamada, K., Abe, K., Li, T., Onoe, Y., Tada, H., Tatsugami, K., Ando, T., Kimura, G., & Nomoto, K. (1998). Characterization of B16 melanoma-specific cytotoxic T lymphocytes. Cancer Immunology Immunotherapy, 47(4), 198-204. https://doi.org/10.1007/s002620050521