雄性不稔スギ爽春の原因遺伝子候補の特徴と花粉形成期に認められた糖代謝異常

Translated title of the contribution: Characterization of candidate gene and abnormal of carbohydrate metabolism during pollen development in a male sterility clone, sosyun

Nanako Yamasaki, Taisei Horiba, Miho Tamura, Noritsugu Kuramoto, Kuniyoshi Shimizu, Atsushi Watanabe

Research output: Contribution to journalArticlepeer-review

Abstract

In recent years, candidate regions containing causative genes of Sosyun, a male sterility trait, have been identified by a linkage map in Cryptomeria japonica with quantitative trait loci analysis. Of which, reCj19250 was found to be closely linked to the causative gene in this candidate region. Another gene called reCj11611, which is presumed to be related to carbohydrate metabolism, was mapped to the same locus as reCj19250, but no details about this gene have been reported to date. In this study, the gene expression of both reCj11611 and reCj19250, or the monosaccharide and starch content, were investigated in C. japonica clones during pollen formation. The monosaccharide or starch content during pollen formation increased significantly in Sosyun, suggesting that carbohydrate metabolism is abnormal in plants with this trait. However, evidence that gene expression of reCj11611 is related to abnormal carbohydrate metabolism was not obtained. A specific mutation of the reCj11611 cDNA sequence associated with Sosyun was determined. Deletion of 20 bps sequences in the 3′UTR region for the reCj11611 cDNA sequence was applied to approximately 760 C. japonica clones using a DNA marker which was able to identify this deletion. Although six clones were shown to be heterozygotic for this gene, five of them were incongruent with the presence of reCj19250. Therefore, the genes cited to be in the same position on the C. japonica linkage map were, in fact, at neighboring loci.

Translated title of the contributionCharacterization of candidate gene and abnormal of carbohydrate metabolism during pollen development in a male sterility clone, sosyun
Original languageJapanese
Pages (from-to)191-197
Number of pages7
JournalNihon Ringakkai Shi/Journal of the Japanese Forestry Society
Volume102
Issue number3
DOIs
Publication statusPublished - Jun 1 2020

All Science Journal Classification (ASJC) codes

  • Forestry

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