Characterization of CD28-CD4+ T cells in living kidney transplant patients with long-term allograft acceptance

Masashi Kato, Tetsuya Matsuguchi, Yoshinari Ono, Ryohei Hattori, Shinichi Ohshima, Yasunobu Yoshikai

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    Abstract

    CD28-CD4+ T-cell subpopulation is expanded in kidney allograft patients with long graft survival. To seek for the roles of CD28-CD4+ T cells in the long-term acceptance of kidney allografts, we characterized this population by analyzing cell surface molecules, TCR VΒ repertoire, mixed lymphocyte reaction (MLR), and cytokine production. The number of CD28-CD4+ T cells increased correlatively with time after transplantation in this group of patients. The CD28-CD4+ T cells did not express detectable levels of CD25, CD69, Vα24, or CTLA-4 but expressed heterogeneous amounts of CD45 RA on the surface. Freshly sorted CD28-CD4+ T cells revealed a restricted Vβ repertoire, whereas the Vβ usage of CD28+-CD4+ T cells from the same patients was much diversified. Expression levels of TGF-β and IFNγ gene were significantly higher in the CD28- CD4+ T cells than in the CD28+CD4+ T cells from the kidney allograft patients. These findings suggest that an oligoclonal CD28- CD4+ T-cell population is continuously activated in patients with long allograft survival, which may be linked with the long-term acceptance. Human Immunology 62, 1335-1345 (2001).

    Original languageEnglish
    Pages (from-to)1335-1345
    Number of pages11
    JournalHuman Immunology
    Volume62
    Issue number12
    DOIs
    Publication statusPublished - Dec 17 2001

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    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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