Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans

H. Aoki, S. Sato, T. Takanami, Takeshi Ishihara, I. Katsura, H. Takahashi, A. Higashitani

Research output: Contribution to journalArticle

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Abstract

An ATM-like gene was identified in the genome of Caenorhabditis elegans. The putative product of the gene, termed Ce-atl-1 (C. elegans ATM-like 1) consists of 2514 amino acid residues. The C-terminal sequence, which contains a PI-3 kinase-like domain, showed good homology with the products of the gene MEC1/ESR1 from budding yeast, the rad3+ gene of fission yeast and mammalian ATR (ataxia-telangiectasia and rad3+ related) genes. The results of RNA-mediated interference indicated that the major phenotype associated with repression of Ce-atl-1 was lethality (approximately 50-80%) during early embryogenesis. Among the surviving progeny, males (XO animals) arose at a high frequency (2-30%). In addition, 5% of oocyte chromosomes demonstrated aneuploidy due to a defect in pre-meiotic chromosomal segregation. Gene expression analyses indicated that Ce-atl-1 mRNA was expressed in all larval stages and that its level increased about fivefold in the adult stage. The adult expression level was decreased in the glp-4 mutant, which is defective in germ line proliferation. Ce-atl-1 was strongly expressed in both the mitotic and meiotic cells of adult gonads. In summary, Ce-atl-1 appears to be important for early embryogenesis, and loss of its function results in a defect in chromosome segregation, similar to what has been observed for AT-related proteins.

Original languageEnglish
Pages (from-to)119-126
Number of pages8
JournalMolecular and General Genetics
Volume264
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

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Caenorhabditis elegans
Genes
Embryonic Development
Ataxia Telangiectasia
Chromosome Segregation
Chromosomes, Human, Pair 5
Saccharomycetales
Schizosaccharomyces
Gonads
Aneuploidy
RNA Interference
Phosphatidylinositol 3-Kinases
Germ Cells
Oocytes
Genome
Phenotype
Gene Expression
Amino Acids
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Aoki, H., Sato, S., Takanami, T., Ishihara, T., Katsura, I., Takahashi, H., & Higashitani, A. (2000). Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans. Molecular and General Genetics, 264(1-2), 119-126. https://doi.org/10.1007/s004380000291

Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans. / Aoki, H.; Sato, S.; Takanami, T.; Ishihara, Takeshi; Katsura, I.; Takahashi, H.; Higashitani, A.

In: Molecular and General Genetics, Vol. 264, No. 1-2, 01.01.2000, p. 119-126.

Research output: Contribution to journalArticle

Aoki, H, Sato, S, Takanami, T, Ishihara, T, Katsura, I, Takahashi, H & Higashitani, A 2000, 'Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans', Molecular and General Genetics, vol. 264, no. 1-2, pp. 119-126. https://doi.org/10.1007/s004380000291
Aoki, H. ; Sato, S. ; Takanami, T. ; Ishihara, Takeshi ; Katsura, I. ; Takahashi, H. ; Higashitani, A. / Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans. In: Molecular and General Genetics. 2000 ; Vol. 264, No. 1-2. pp. 119-126.
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