Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors

Saori Yamaguchi, Tomotoshi Marumoto, Takenobu Nii, Hirotaka Kawano, Jiyuan Liao, Yoko Nagai, Michiyo Okada, Atsushi Takahashi, Hiroyuki Inoue, Erika Sasaki, Hiroshi Fujii, Shinji Okano, Hayao Ebise, Tetsuya Sato, Mikita Suyama, Hideyuki Okano, Yoshie Miura, Kenzaburo Tani

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Recent generation of induced pluripotent stem (iPSCs) has made a significant impact on the field of human regenerative medicine. Prior to the clinical application of iPSCs, testing of their safety and usefulness must be carried out using reliable animal models of various diseases. In order to generate iPSCs from common marmoset (CM; Callithrix jacchus), one of the most useful experimental animals, we have lentivirally transduced reprogramming factors, including POU5F1 (also known as OCT3/4), SOX2, KLF4, and c-MYC into CM fibroblasts. The cells formed round colonies expressing embryonic stem cell markers, however, they showed an abnormal karyotype denoted as 46, X, del(4q), +mar, and formed human dysgerminoma-like tumors in SCID mice, indicating that the transduction of reprogramming factors caused unexpected tumorigenesis of CM cells. Moreover, CM dysgerminoma-like tumors were highly sensitive to DNA-damaging agents, irradiation, and fibroblast growth factor receptor inhibitor, and their growth was dependent on c-MYC expression. These results indicate that DNA-damaging agents, irradiation, fibroblast growth factor receptor inhibitor, and c-MYC-targeted therapies might represent effective treatment strategies for unexpected tumors in patients receiving iPSC-based therapy. Reprogramming factors for iPSC generation can be oncogenic, and thus reprogramming factor transduced cells might have tumorigenic potential. Here we characterized common marmoset dysgerminoma like tumor, CM DGs, generated by the lentivirally transduced reprogramming factors into fibroblasts. The growth of CM DGs was dependent on c-MYC expression and bFGF signaling. Moreover CM DGs were highly sensitive to DNA damaging agents, irradiation and FGFR inhibitors. Therefore irradiation, DNA damaging agents and FGFR inhibitors might be effective for controlling reprogramming factor related tumors that may be found in patients treated with iPSC-based medicine.

    Original languageEnglish
    Pages (from-to)402-408
    Number of pages7
    JournalCancer Science
    Volume105
    Issue number4
    DOIs
    Publication statusPublished - Jan 1 2014

    Fingerprint

    Dysgerminoma
    Callithrix
    Fibroblast Growth Factor Receptors
    DNA
    Neoplasms
    Fibroblasts
    Abnormal Karyotype
    Mars
    Animal Disease Models
    Growth Inhibitors
    Regenerative Medicine
    SCID Mice
    Embryonic Stem Cells
    Carcinogenesis
    Therapeutics
    Medicine
    Safety
    Growth

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

    Cite this

    Yamaguchi, S., Marumoto, T., Nii, T., Kawano, H., Liao, J., Nagai, Y., ... Tani, K. (2014). Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors. Cancer Science, 105(4), 402-408. https://doi.org/10.1111/cas.12367

    Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors. / Yamaguchi, Saori; Marumoto, Tomotoshi; Nii, Takenobu; Kawano, Hirotaka; Liao, Jiyuan; Nagai, Yoko; Okada, Michiyo; Takahashi, Atsushi; Inoue, Hiroyuki; Sasaki, Erika; Fujii, Hiroshi; Okano, Shinji; Ebise, Hayao; Sato, Tetsuya; Suyama, Mikita; Okano, Hideyuki; Miura, Yoshie; Tani, Kenzaburo.

    In: Cancer Science, Vol. 105, No. 4, 01.01.2014, p. 402-408.

    Research output: Contribution to journalArticle

    Yamaguchi, S, Marumoto, T, Nii, T, Kawano, H, Liao, J, Nagai, Y, Okada, M, Takahashi, A, Inoue, H, Sasaki, E, Fujii, H, Okano, S, Ebise, H, Sato, T, Suyama, M, Okano, H, Miura, Y & Tani, K 2014, 'Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors', Cancer Science, vol. 105, no. 4, pp. 402-408. https://doi.org/10.1111/cas.12367
    Yamaguchi, Saori ; Marumoto, Tomotoshi ; Nii, Takenobu ; Kawano, Hirotaka ; Liao, Jiyuan ; Nagai, Yoko ; Okada, Michiyo ; Takahashi, Atsushi ; Inoue, Hiroyuki ; Sasaki, Erika ; Fujii, Hiroshi ; Okano, Shinji ; Ebise, Hayao ; Sato, Tetsuya ; Suyama, Mikita ; Okano, Hideyuki ; Miura, Yoshie ; Tani, Kenzaburo. / Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors. In: Cancer Science. 2014 ; Vol. 105, No. 4. pp. 402-408.
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