Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.

H. Urata, H. Shimizu, H. Hiroaki, Daisuke Kohda, M. Akagi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to investigate the duplex structure of DNA and RNA containing an L-nucleotide residue, we carried out the UV-melting and CD experiments. Although the introduction of an L-nucleotide into DNA/DNA, DNA/RNA and RNA/RNA duplexes reduced their Tm values, the typical two-state transitions were observed for all of the duplexes. The incorporation of an L-nucleotide into the RNA strand caused more remarkable decreases of the Tm value than that into the DNA strand. The CD spectra of the DNA/DNA and RNA/RNA duplexes containing an L-nucleotide are similar to those of unmodified duplexes. On the other hand, the spectra of the heterochiral DNA/RNA hybrid duplexes are significantly different from those of the homochiral DNA/RNA hybrid duplexes, which are generally thought to adopt the A-form.

Original languageEnglish
Pages (from-to)243-244
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

Nucleotides
RNA
DNA
Freezing

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide. / Urata, H.; Shimizu, H.; Hiroaki, H.; Kohda, Daisuke; Akagi, M.

In: Nucleic acids research. Supplement (2001), No. 1, 01.01.2001, p. 243-244.

Research output: Contribution to journalArticle

@article{06790359ce354596b9a0d77c15209920,
title = "Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.",
abstract = "In order to investigate the duplex structure of DNA and RNA containing an L-nucleotide residue, we carried out the UV-melting and CD experiments. Although the introduction of an L-nucleotide into DNA/DNA, DNA/RNA and RNA/RNA duplexes reduced their Tm values, the typical two-state transitions were observed for all of the duplexes. The incorporation of an L-nucleotide into the RNA strand caused more remarkable decreases of the Tm value than that into the DNA strand. The CD spectra of the DNA/DNA and RNA/RNA duplexes containing an L-nucleotide are similar to those of unmodified duplexes. On the other hand, the spectra of the heterochiral DNA/RNA hybrid duplexes are significantly different from those of the homochiral DNA/RNA hybrid duplexes, which are generally thought to adopt the A-form.",
author = "H. Urata and H. Shimizu and H. Hiroaki and Daisuke Kohda and M. Akagi",
year = "2001",
month = "1",
day = "1",
doi = "10.1093/nass/1.1.243",
language = "English",
pages = "243--244",
journal = "Nucleic acids research. Supplement (2001)",
number = "1",

}

TY - JOUR

T1 - Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.

AU - Urata, H.

AU - Shimizu, H.

AU - Hiroaki, H.

AU - Kohda, Daisuke

AU - Akagi, M.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - In order to investigate the duplex structure of DNA and RNA containing an L-nucleotide residue, we carried out the UV-melting and CD experiments. Although the introduction of an L-nucleotide into DNA/DNA, DNA/RNA and RNA/RNA duplexes reduced their Tm values, the typical two-state transitions were observed for all of the duplexes. The incorporation of an L-nucleotide into the RNA strand caused more remarkable decreases of the Tm value than that into the DNA strand. The CD spectra of the DNA/DNA and RNA/RNA duplexes containing an L-nucleotide are similar to those of unmodified duplexes. On the other hand, the spectra of the heterochiral DNA/RNA hybrid duplexes are significantly different from those of the homochiral DNA/RNA hybrid duplexes, which are generally thought to adopt the A-form.

AB - In order to investigate the duplex structure of DNA and RNA containing an L-nucleotide residue, we carried out the UV-melting and CD experiments. Although the introduction of an L-nucleotide into DNA/DNA, DNA/RNA and RNA/RNA duplexes reduced their Tm values, the typical two-state transitions were observed for all of the duplexes. The incorporation of an L-nucleotide into the RNA strand caused more remarkable decreases of the Tm value than that into the DNA strand. The CD spectra of the DNA/DNA and RNA/RNA duplexes containing an L-nucleotide are similar to those of unmodified duplexes. On the other hand, the spectra of the heterochiral DNA/RNA hybrid duplexes are significantly different from those of the homochiral DNA/RNA hybrid duplexes, which are generally thought to adopt the A-form.

UR - http://www.scopus.com/inward/record.url?scp=0042203685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042203685&partnerID=8YFLogxK

U2 - 10.1093/nass/1.1.243

DO - 10.1093/nass/1.1.243

M3 - Article

C2 - 12836355

AN - SCOPUS:0042203685

SP - 243

EP - 244

JO - Nucleic acids research. Supplement (2001)

JF - Nucleic acids research. Supplement (2001)

IS - 1

ER -