Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.

H. Urata, H. Shimizu, H. Hiroaki, D. Kohda, M. Akagi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In order to investigate the duplex structure of DNA and RNA containing an L-nucleotide residue, we carried out the UV-melting and CD experiments. Although the introduction of an L-nucleotide into DNA/DNA, DNA/RNA and RNA/RNA duplexes reduced their Tm values, the typical two-state transitions were observed for all of the duplexes. The incorporation of an L-nucleotide into the RNA strand caused more remarkable decreases of the Tm value than that into the DNA strand. The CD spectra of the DNA/DNA and RNA/RNA duplexes containing an L-nucleotide are similar to those of unmodified duplexes. On the other hand, the spectra of the heterochiral DNA/RNA hybrid duplexes are significantly different from those of the homochiral DNA/RNA hybrid duplexes, which are generally thought to adopt the A-form.

Original languageEnglish
Pages (from-to)243-244
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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