Characterization of enzymatically gellable, phenolated linear poly(ethylene glycol) with different molecular weights for encapsulating living cells

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6 Citations (Scopus)

Abstract

Enzymatic hydrogelation has received much attention due to the high biocompatibility and the ease of control of reaction kinetics under physiological conditions. In particular, horseradish peroxidase (HRP)-mediated phenol coupling reaction has great potential for developing in situ hydrogelation systems. Herein, we report the HRP-catalyzed preparation and characterization of hydrogels composed of a terminally bis-phenolated linear poly(ethylene glycol) (PEG-Ph-OH) with different molecular weights (Mws 3100, 8800, 11,000, 20,000g/mol). The gelation time of polymer solution can be controlled in the range from few second to few minute, suggestion that the PEG-Ph-OH has a potential as a in situ forming hydrogel. In addition, the physicochemical properties of the hydrogels, such as swelling ratio, mesh size and mechanical property, were controlled by the molecular weight of the PEG-Ph-OH. The results could be attributed to the alteration in the cross-linking density by the variation of molecular weight of the gel precursor. Furthermore, the viability of mammalian cells encapsulated in the PEG-Ph-OH hydrogels was approximately 90%. These results indicate that PEG-Ph-OH has potential for biomedical applications including tissue engineering.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalBiochemical Engineering Journal
Volume93
DOIs
Publication statusPublished - Sep 6 2014

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Ethylene Glycol
Polyethylene glycols
Molecular Weight
Molecular weight
Cells
Hydrogels
Horseradish Peroxidase
Hydrogel
Tissue Engineering
Phenol
Bioelectric potentials
Gelation
Polymer solutions
Cell Survival
Biocompatibility
Polymers
Tissue engineering
Reaction kinetics
Phenols
Gels

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

Cite this

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title = "Characterization of enzymatically gellable, phenolated linear poly(ethylene glycol) with different molecular weights for encapsulating living cells",
abstract = "Enzymatic hydrogelation has received much attention due to the high biocompatibility and the ease of control of reaction kinetics under physiological conditions. In particular, horseradish peroxidase (HRP)-mediated phenol coupling reaction has great potential for developing in situ hydrogelation systems. Herein, we report the HRP-catalyzed preparation and characterization of hydrogels composed of a terminally bis-phenolated linear poly(ethylene glycol) (PEG-Ph-OH) with different molecular weights (Mws 3100, 8800, 11,000, 20,000g/mol). The gelation time of polymer solution can be controlled in the range from few second to few minute, suggestion that the PEG-Ph-OH has a potential as a in situ forming hydrogel. In addition, the physicochemical properties of the hydrogels, such as swelling ratio, mesh size and mechanical property, were controlled by the molecular weight of the PEG-Ph-OH. The results could be attributed to the alteration in the cross-linking density by the variation of molecular weight of the gel precursor. Furthermore, the viability of mammalian cells encapsulated in the PEG-Ph-OH hydrogels was approximately 90{\%}. These results indicate that PEG-Ph-OH has potential for biomedical applications including tissue engineering.",
author = "Kousuke Moriyama and Rie Wakabayashi and Masahiro Goto and Noriho Kamiya",
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AU - Moriyama, Kousuke

AU - Wakabayashi, Rie

AU - Goto, Masahiro

AU - Kamiya, Noriho

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AB - Enzymatic hydrogelation has received much attention due to the high biocompatibility and the ease of control of reaction kinetics under physiological conditions. In particular, horseradish peroxidase (HRP)-mediated phenol coupling reaction has great potential for developing in situ hydrogelation systems. Herein, we report the HRP-catalyzed preparation and characterization of hydrogels composed of a terminally bis-phenolated linear poly(ethylene glycol) (PEG-Ph-OH) with different molecular weights (Mws 3100, 8800, 11,000, 20,000g/mol). The gelation time of polymer solution can be controlled in the range from few second to few minute, suggestion that the PEG-Ph-OH has a potential as a in situ forming hydrogel. In addition, the physicochemical properties of the hydrogels, such as swelling ratio, mesh size and mechanical property, were controlled by the molecular weight of the PEG-Ph-OH. The results could be attributed to the alteration in the cross-linking density by the variation of molecular weight of the gel precursor. Furthermore, the viability of mammalian cells encapsulated in the PEG-Ph-OH hydrogels was approximately 90%. These results indicate that PEG-Ph-OH has potential for biomedical applications including tissue engineering.

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