Characterization of evolutionary trend in squamate estrogen receptor sensitivity

Ryohei Yatsu, Yoshinao Katsu, Satomi Kohno, Takeshi Mizutani, Yukiko Ogino, Yasuhiko Ohta, Jan Myburgh, Johannes H. van Wyk, Louis J. Guillette, Shinichi Miyagawa, Taisen Iguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Steroid hormones are a key regulator of reproductive biology in vertebrates, and are largely regulated via nuclear receptor families. Estrogen signaling is regulated by two estrogen receptor (ER) subtypes alpha and beta in the nucleus. In order to understand the role of estrogen in vertebrates, these ER from various species have been isolated and were functionally analyzed using luciferase reporter gene assays. Interestingly, species difference in estrogen sensitivity has been noted in the past, and it was reported that snake ER displayed highest estrogen sensitivity. Here, we isolated additional ER from three lizards: chameleon (Bradypodion pumilum), skink (Plestiodon finitimus), and gecko (Gekko japonicus). We have performed functional characterization of these ERs using reporter gene assay system, and found high estrogen sensitivity in all three species. Furthermore, comparison with results from other tetrapod ER revealed a seemingly uniform gradual pattern of ligand sensitivity evolution. In silico 3D homology modeling of the ligand-binding domain revealed structural variation at three sites, helix 2, and juncture between helices 8 and 9, and caudal region of helix 10/11. Docking simulations indicated that predicted ligand-receptor interaction also correlated with the reporter assay results, and overall squamates displayed highest stabilized interactions. The assay system and homology modeling system provides tool for in-depth comparative analysis of estrogen function, and provides insight toward the evolution of ER among vertebrates.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume238
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

Squamata
Estrogen Receptors
estrogens
Estrogens
Lizards
Vertebrates
vertebrates
assays
Ligands
Reporter Genes
reporter genes
Estrogen Receptor beta
receptors
Snakes
Estrogen Receptor alpha
Scincidae
Gekkonidae
steroid hormones
luciferase
Cytoplasmic and Nuclear Receptors

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Endocrinology

Cite this

Characterization of evolutionary trend in squamate estrogen receptor sensitivity. / Yatsu, Ryohei; Katsu, Yoshinao; Kohno, Satomi; Mizutani, Takeshi; Ogino, Yukiko; Ohta, Yasuhiko; Myburgh, Jan; van Wyk, Johannes H.; Guillette, Louis J.; Miyagawa, Shinichi; Iguchi, Taisen.

In: General and Comparative Endocrinology, Vol. 238, 01.11.2016, p. 88-95.

Research output: Contribution to journalArticle

Yatsu, R, Katsu, Y, Kohno, S, Mizutani, T, Ogino, Y, Ohta, Y, Myburgh, J, van Wyk, JH, Guillette, LJ, Miyagawa, S & Iguchi, T 2016, 'Characterization of evolutionary trend in squamate estrogen receptor sensitivity', General and Comparative Endocrinology, vol. 238, pp. 88-95. https://doi.org/10.1016/j.ygcen.2016.04.005
Yatsu, Ryohei ; Katsu, Yoshinao ; Kohno, Satomi ; Mizutani, Takeshi ; Ogino, Yukiko ; Ohta, Yasuhiko ; Myburgh, Jan ; van Wyk, Johannes H. ; Guillette, Louis J. ; Miyagawa, Shinichi ; Iguchi, Taisen. / Characterization of evolutionary trend in squamate estrogen receptor sensitivity. In: General and Comparative Endocrinology. 2016 ; Vol. 238. pp. 88-95.
@article{a3bdbbd894ca439ab0b576e41dcaeb2a,
title = "Characterization of evolutionary trend in squamate estrogen receptor sensitivity",
abstract = "Steroid hormones are a key regulator of reproductive biology in vertebrates, and are largely regulated via nuclear receptor families. Estrogen signaling is regulated by two estrogen receptor (ER) subtypes alpha and beta in the nucleus. In order to understand the role of estrogen in vertebrates, these ER from various species have been isolated and were functionally analyzed using luciferase reporter gene assays. Interestingly, species difference in estrogen sensitivity has been noted in the past, and it was reported that snake ER displayed highest estrogen sensitivity. Here, we isolated additional ER from three lizards: chameleon (Bradypodion pumilum), skink (Plestiodon finitimus), and gecko (Gekko japonicus). We have performed functional characterization of these ERs using reporter gene assay system, and found high estrogen sensitivity in all three species. Furthermore, comparison with results from other tetrapod ER revealed a seemingly uniform gradual pattern of ligand sensitivity evolution. In silico 3D homology modeling of the ligand-binding domain revealed structural variation at three sites, helix 2, and juncture between helices 8 and 9, and caudal region of helix 10/11. Docking simulations indicated that predicted ligand-receptor interaction also correlated with the reporter assay results, and overall squamates displayed highest stabilized interactions. The assay system and homology modeling system provides tool for in-depth comparative analysis of estrogen function, and provides insight toward the evolution of ER among vertebrates.",
author = "Ryohei Yatsu and Yoshinao Katsu and Satomi Kohno and Takeshi Mizutani and Yukiko Ogino and Yasuhiko Ohta and Jan Myburgh and {van Wyk}, {Johannes H.} and Guillette, {Louis J.} and Shinichi Miyagawa and Taisen Iguchi",
year = "2016",
month = "11",
day = "1",
doi = "10.1016/j.ygcen.2016.04.005",
language = "English",
volume = "238",
pages = "88--95",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Characterization of evolutionary trend in squamate estrogen receptor sensitivity

AU - Yatsu, Ryohei

AU - Katsu, Yoshinao

AU - Kohno, Satomi

AU - Mizutani, Takeshi

AU - Ogino, Yukiko

AU - Ohta, Yasuhiko

AU - Myburgh, Jan

AU - van Wyk, Johannes H.

AU - Guillette, Louis J.

AU - Miyagawa, Shinichi

AU - Iguchi, Taisen

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Steroid hormones are a key regulator of reproductive biology in vertebrates, and are largely regulated via nuclear receptor families. Estrogen signaling is regulated by two estrogen receptor (ER) subtypes alpha and beta in the nucleus. In order to understand the role of estrogen in vertebrates, these ER from various species have been isolated and were functionally analyzed using luciferase reporter gene assays. Interestingly, species difference in estrogen sensitivity has been noted in the past, and it was reported that snake ER displayed highest estrogen sensitivity. Here, we isolated additional ER from three lizards: chameleon (Bradypodion pumilum), skink (Plestiodon finitimus), and gecko (Gekko japonicus). We have performed functional characterization of these ERs using reporter gene assay system, and found high estrogen sensitivity in all three species. Furthermore, comparison with results from other tetrapod ER revealed a seemingly uniform gradual pattern of ligand sensitivity evolution. In silico 3D homology modeling of the ligand-binding domain revealed structural variation at three sites, helix 2, and juncture between helices 8 and 9, and caudal region of helix 10/11. Docking simulations indicated that predicted ligand-receptor interaction also correlated with the reporter assay results, and overall squamates displayed highest stabilized interactions. The assay system and homology modeling system provides tool for in-depth comparative analysis of estrogen function, and provides insight toward the evolution of ER among vertebrates.

AB - Steroid hormones are a key regulator of reproductive biology in vertebrates, and are largely regulated via nuclear receptor families. Estrogen signaling is regulated by two estrogen receptor (ER) subtypes alpha and beta in the nucleus. In order to understand the role of estrogen in vertebrates, these ER from various species have been isolated and were functionally analyzed using luciferase reporter gene assays. Interestingly, species difference in estrogen sensitivity has been noted in the past, and it was reported that snake ER displayed highest estrogen sensitivity. Here, we isolated additional ER from three lizards: chameleon (Bradypodion pumilum), skink (Plestiodon finitimus), and gecko (Gekko japonicus). We have performed functional characterization of these ERs using reporter gene assay system, and found high estrogen sensitivity in all three species. Furthermore, comparison with results from other tetrapod ER revealed a seemingly uniform gradual pattern of ligand sensitivity evolution. In silico 3D homology modeling of the ligand-binding domain revealed structural variation at three sites, helix 2, and juncture between helices 8 and 9, and caudal region of helix 10/11. Docking simulations indicated that predicted ligand-receptor interaction also correlated with the reporter assay results, and overall squamates displayed highest stabilized interactions. The assay system and homology modeling system provides tool for in-depth comparative analysis of estrogen function, and provides insight toward the evolution of ER among vertebrates.

UR - http://www.scopus.com/inward/record.url?scp=84992188050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992188050&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2016.04.005

DO - 10.1016/j.ygcen.2016.04.005

M3 - Article

C2 - 27072832

AN - SCOPUS:84992188050

VL - 238

SP - 88

EP - 95

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

ER -