Characterization of flagellar antigens and insecticidal activities of Bacillus thuringiensis populations in animal feces

Dong Hyun Lee, Noriko Shisa, Naoya Wasano, Akira Ohgushi, Michio Ohba

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

In total, 287 Bacillus thuringiensis isolates, recovered from feces of 28 zoo-maintained animal species, were examined for flagellar (H) antigenicity and insecticidal activity. Serologically, 209 isolates (72.8%) were allocated to the 8 H serogroups, 4 were untypable, and 74 were untestable. Among the 8 H serotypes detected, H3abc (serovar kurstaki) predominated at a high frequency of 88.0%, followed by H6 (serovar entomocidus) with a frequency of 7.7%. Insecticidal activity was associated with 67.2% of the fecal populations: 188 isolates were toxic to both Bombyx mori (Lepidoptera: Bombycidae) and Aedes aegypti (Diptera: Culicidae), 2 isolates were specific for B. mori, and 3 isolates were toxic to A. aegypti only. Of the isolates with dual toxicity, 97.9% belonged to the serovar kurstaki, producing bipyramidal parasporal inclusions. All of the H7 (serovar aizawai) isolates were toxic to both insects.

Original languageEnglish
Pages (from-to)287-290
Number of pages4
JournalCurrent Microbiology
Volume46
Issue number4
DOIs
Publication statusPublished - Apr 2003

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Characterization of flagellar antigens and insecticidal activities of Bacillus thuringiensis populations in animal feces'. Together they form a unique fingerprint.

Cite this