Characterization of Functional Domains of the Hemolytic Lectin CEL-III from the Marine Invertebrate Cucumaria echinata

Yoshiaki Kouzuma, Yota Suzuki, Masahiro Nakano, Kayo Matsuyama, Sumiki Tojo, Makoto Kimura, Takayuki Yamasaki, Haruhiko Aoyagi, Tomomitsu Hatakeyama

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Abstract

CEL-III is a Ca2+-dependent, galactose/N-acetylgalactosamine (GalNAc)-specific lectin isolated from the marine invertebrate Cucumaria echinata. This lectin exhibits strong hemolytic activity and cytotoxicity through pore formation in target cell membranes. The amino acid sequence of CEL-III revealed the N-terminal two-thirds to have homology to the B-chains of ricin and abrin, which are galactose-specific plant toxic lectins; the C-terminal one-third shows no homology to any known proteins. To examine the carbohydrate-binding ability of the N-terminal region of CEL-III, the protein comprising Pyr1-Phe283 was expressed in Escherichia coli cells. The expressed protein showed both the ability to bind to a GalNAc-immobilized column as well as hemagglutinating activity for rabbit erythrocytes, confirming that the N-terminal region has binding activity for specific carbohydrates. Since the C-terminal region could not be expressed in E. coli cells, a fragment containing this region was produced by limited proteolysis of the native protein by trypsin. The resulting C-terminal 15 kDa fragment of CEL-III exhibited a tendency to self-associate, forming an oligomer. When mixed with erythrocytes, the oligomer of the C-terminal fragment caused hemagglutination, probably due to hydrophobic interaction with cell membranes, while the monomeric fragment did not. Chymotryptic digestion of the preformed CEL-III oligomer induced upon lactose binding also yielded an oligomer of the C-terminal fragment comprising six molecules of the 16 kDa fragment. These results suggest that after binding to cell surface carbohydrate chains, CEL-III oligomerizes through C-terminal domains, leading to the formation of ion-permeable pores by hydrophobic interaction with the cell membrane.

Original languageEnglish
Pages (from-to)395-402
Number of pages8
JournalJournal of biochemistry
Volume134
Issue number3
DOIs
Publication statusPublished - Sep 2003

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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    Kouzuma, Y., Suzuki, Y., Nakano, M., Matsuyama, K., Tojo, S., Kimura, M., Yamasaki, T., Aoyagi, H., & Hatakeyama, T. (2003). Characterization of Functional Domains of the Hemolytic Lectin CEL-III from the Marine Invertebrate Cucumaria echinata. Journal of biochemistry, 134(3), 395-402. https://doi.org/10.1093/jb/mvg157