Characterization of genome-reduced fission yeast strains

Mayumi Sasaki, Hiromichi Kumagai, Kaoru Takegawa, Hideki Tohda

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the left arm of chromosome I, 155.4 kb in the right arm of chromosome I, 211.7 kb in the left arm of chromosome II and 121.6 kb in the right arm of chromosome II. The deletions corresponded to a loss of 223 genes of the original ∼5100. The quadruple-deletion strain, with a total deletion size of 657.3 kb, showed a decreased ability to uptake glucose and some amino acids in comparison with the parental strain. The strain also showed increased gene expression of the mating pheromone M-factor precursor and the nicotinamide adenine dinucleotide phosphate -specific glutamate dehydrogenase. There was also a 2.7-fold increase in the concentration of cellular adenosine triphosphate, and levels of the heterologous proteins, enhanced green fluorescent protein and secreted human growth hormone were increased by 1.7- and 1.8-fold, respectively. The transcriptome data from this study have been submitted to the Gene Expression Omnibus (GEO: http://www.ncbi.nlm.nih.gov/geo/) under the accession number GSE38620 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token= vjkxjewuywgcovc&acc=GSE38620).

Original languageEnglish
Pages (from-to)5382-5399
Number of pages18
JournalNucleic acids research
Volume41
Issue number10
DOIs
Publication statusPublished - May 2013

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'Characterization of genome-reduced fission yeast strains'. Together they form a unique fingerprint.

Cite this