Characterization of glutamate receptors induced in Xenopus oocytes after injection of rat brain mRNA

Chikara Hirono, Isao Ito, Shunichi Yamagishi, Hiroyuki Sugiyama

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Xenopus oocytes in which poly(A)+ mRNA isolated from rat brains were previously injected, exhibited at least 3 categories of current responses to excitatory amino acids. They were oscillatory responses to glutamate (Glu) or quisqualate (QA), smooth large responses to kainate (KA), and smooth small responses to Glu and QA. Oscillatory responses were mediated by a metabotropic type of Glu receptor which is coupled to a G-protein but not directly to an ionic channel. Amplitudes of smooth Glu responses and smooth QA responses were similar in size, and were not additive to each other, suggesting a common receptor mediating both responses. l-Glutamylglycine inhibited KA responses in a competitive manner without affecting smooth Glu/QA responses, indicating that KA and smooth Glu/QA responses were mediated by separate receptors. From these results, it was concluded that the injection of rat brain mRNA induced at least 3 different glutamatergic receptors: receptors mediating (a) KA responses and (b) smooth Glu/QA responses, and (c) the metabotropic Glu receptor. The former two may most likely correspond to Glu receptor subtypes preferring KA and QA, respectively, as seen in the brain.

Original languageEnglish
Pages (from-to)106-114
Number of pages9
JournalNeuroscience Research
Volume6
Issue number2
DOIs
Publication statusPublished - Dec 1988

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Quisqualic Acid
Glutamate Receptors
Xenopus
Oocytes
Kainic Acid
Glutamic Acid
Messenger RNA
Injections
Brain
Metabotropic Glutamate Receptors
Excitatory Amino Acids
Ion Channels
GTP-Binding Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Characterization of glutamate receptors induced in Xenopus oocytes after injection of rat brain mRNA. / Hirono, Chikara; Ito, Isao; Yamagishi, Shunichi; Sugiyama, Hiroyuki.

In: Neuroscience Research, Vol. 6, No. 2, 12.1988, p. 106-114.

Research output: Contribution to journalArticle

Hirono, Chikara ; Ito, Isao ; Yamagishi, Shunichi ; Sugiyama, Hiroyuki. / Characterization of glutamate receptors induced in Xenopus oocytes after injection of rat brain mRNA. In: Neuroscience Research. 1988 ; Vol. 6, No. 2. pp. 106-114.
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