Characterization of immunoglobulin light chain isotypes in the common carp

Mitsuru Tomana, Jun Ishikawa, Etsuou Imai, Tadaaki Moritomo, Miki Nakao, Tomoki Yano

Research output: Contribution to journalArticle

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Abstract

Two or three isotypes of immunoglobulin (Ig) light (L) chain have been demonstrated in several teleost species thus far. A reverse transcription-polymerase chain reaction (RT-PCR) strategy was used with the aim of isolating cDNA clones encoding IgL chain from the common carp (Cyprinus carpio. L.) different from the previously isolated isotype, designated L1A. cDNA clones representing two novel isotypes, designated L1B and L3, were obtained in this study. For CL segments, L1B sequences were similar to type L1/G in various teleost species, as well as L1A, while L3 sequences were closely related with type L3/F in catfish and zebrafish. For VL segments, however, L1A sequences demonstrated higher similarity to L3 rather than L1B. These results were also supported by the phylogenetic study. The similarity found between L1A and L3 VL sequences was based on the very high degree of nucleotide identity in FR1, and/or FR2 to FR3, strongly suggesting the involvement of interlocus recombination between them. Southern blot analyses suggested that the locus of L1B has a cluster-like organization, but that those of L3 and L1A are currently unknown due to cross-hybridization between those VL probes. Northern blot analyses showed that CL mRNA of each isotype was expressed in lymphoid tissues examined, particularly strongly in pro-nephros, and was predominantly composed of approximately 1-kb and 0.6-kb transcripts, possibly corresponding to fully spliced (LVJC) and truncated (JC/J-C) forms, respectively.

Original languageEnglish
Pages (from-to)120-129
Number of pages10
JournalImmunogenetics
Volume54
Issue number2
DOIs
Publication statusPublished - Aug 6 2002

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Immunoglobulin Light Chains
Carps
Complementary DNA
Clone Cells
Catfishes
Lymphoid Tissue
Zebrafish
Southern Blotting
Northern Blotting
Genetic Recombination
Reverse Transcription
Nucleotides
Polymerase Chain Reaction
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Characterization of immunoglobulin light chain isotypes in the common carp. / Tomana, Mitsuru; Ishikawa, Jun; Imai, Etsuou; Moritomo, Tadaaki; Nakao, Miki; Yano, Tomoki.

In: Immunogenetics, Vol. 54, No. 2, 06.08.2002, p. 120-129.

Research output: Contribution to journalArticle

Tomana, M, Ishikawa, J, Imai, E, Moritomo, T, Nakao, M & Yano, T 2002, 'Characterization of immunoglobulin light chain isotypes in the common carp', Immunogenetics, vol. 54, no. 2, pp. 120-129. https://doi.org/10.1007/s00251-002-0447-7
Tomana, Mitsuru ; Ishikawa, Jun ; Imai, Etsuou ; Moritomo, Tadaaki ; Nakao, Miki ; Yano, Tomoki. / Characterization of immunoglobulin light chain isotypes in the common carp. In: Immunogenetics. 2002 ; Vol. 54, No. 2. pp. 120-129.
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