Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T

Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Takumi Mitsudome, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin ichiro Asano, Chisa Yasunaga-Aoki

Research output: Contribution to journalArticle

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Abstract

A scaffold obtained from whole-genome shotgun sequencing of Paenibacillus popilliae ATCC 14706T shares partial homology with plasmids found in other strains of P. popilliae. PCR and sequencing for gap enclosure indicated that the scaffold originated from a 15,929-bp circular DNA. The restriction patterns of a plasmid isolated from P. popilliae ATCC 14706T were identical to those expected from the sequence; thus, this circular DNA was identified as a plasmid of ATCC 14706T and designated pPOP15.9. The plasmid encodes 17 putative open reading frames. Orfs 1, 5, 7, 8, and 9 are homologous to Orfs 11, 12, 15, 16, and 17, respectively. Orf1 and Orf11 are annotated as replication initiation proteins. Orf8 and Orf16 are homologs of KfrA, a plasmid-stabilizing protein in Gram-negative bacteria. Recombinant Orf8 and Orf16 proteins were assessed for the properties of KfrA. Indeed, they formed multimers and bound to inverted repeat sequences in upstream regions of both orf8 and orf16. A phylogenetic tree based on amino acid sequences of Orf8, Orf16 and Kfr proteins did not correlate with species lineage.

Original languageEnglish
Pages (from-to)29-44
Number of pages16
JournalMeta Gene
Volume4
DOIs
Publication statusPublished - Jun 1 2015

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Paenibacillus
Plasmids
Contagious Ecthyma
Circular DNA
Proteins
Inverted Repeat Sequences
Firearms
Gram-Negative Bacteria
Open Reading Frames
Amino Acid Sequence
Genome
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T. / Iiyama, Kazuhiro; Mon, Hiroaki; Mori, Kazuki; Mitsudome, Takumi; Lee, Jae Man; Kusakabe, Takahiro; Tashiro, Kousuke; Asano, Shin ichiro; Yasunaga-Aoki, Chisa.

In: Meta Gene, Vol. 4, 01.06.2015, p. 29-44.

Research output: Contribution to journalArticle

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