Characterization of Lantibiotic Nisin Z Produced by Lactococcus lactis IO-1

Hiromi Matsusaki, Kenji Sonomoto, Ayaaki Ishizaki

Research output: Contribution to journalArticle

Abstract

Lactococcus lactis IO-1 isolated in our laboratory produces a peptide antibiotic, a natural nisin variant, nisin Z. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that molecular weight of nisin Z was almost the same as that of nisin A with 3.4 kDa, while reversed-phase high performance chromatography did apparent differences in elution time. Nisin Z was heat stable at acidic pH and showed bactericidal mode of action against an indicator strain, Bacillus subtilis C1. The effect of proteolytic enzyme treatment on the activity and the antimicrobial spectrum were tested. These indicated nearly the same characteristics as those of nisin A. In this study, it was found that two nisin variants (nisin Z and nisin A) were practically inactivated by proteinase K and actinase E. Interestingly, nisin-producing strains, L. lactis IO-1 and L. lactis NCDO 497 (nisin A producer) were sensitive at a high concentration of nisin variants including their own products. The growth of Gram-negative bacterium, Escherichia coli was also inhibited by high concentration of nisin, although nisin producers and Gram-negative bacteria are generally resistant to nisin variants.

Original languageEnglish
Pages (from-to)181-193
Number of pages13
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume43
Issue number1-2
Publication statusPublished - Nov 1998

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Nisin
Bacteriocins
Lactococcus lactis
nisin
bacteriocins
Gram-Negative Bacteria
Endopeptidase K
nisin Z
Bacillus subtilis
Sodium Dodecyl Sulfate
Gram-negative bacteria
Chromatography
Polyacrylamide Gel Electrophoresis
Peptide Hydrolases
Hot Temperature
Molecular Weight
peptide antibiotics
Escherichia coli
Anti-Bacterial Agents
Peptides

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Characterization of Lantibiotic Nisin Z Produced by Lactococcus lactis IO-1. / Matsusaki, Hiromi; Sonomoto, Kenji; Ishizaki, Ayaaki.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 43, No. 1-2, 11.1998, p. 181-193.

Research output: Contribution to journalArticle

Matsusaki, Hiromi ; Sonomoto, Kenji ; Ishizaki, Ayaaki. / Characterization of Lantibiotic Nisin Z Produced by Lactococcus lactis IO-1. In: Journal of the Faculty of Agriculture, Kyushu University. 1998 ; Vol. 43, No. 1-2. pp. 181-193.
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