Characterization of long-term endogenous cardiac repair in children after heart transplantation

Stefan Rupp, Masamichi Koyanagi, Masayoshi Iwasaki, Jürgen Bauer, Susanne Von Gerlach, Dietmar Schranz, Andreas M. Zeiher, Stefanie Dimmeler

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aims: Circulating cells repopulate the heart at a very low rate in adult humans. The knowledge about time-dependent cardiac regeneration is very limited and the contribution of circulating cells to cardiomyocytes or vascular cells in children is unknown. This study investigates the endogenous repair capacity and the long-term incorporation of circulating cells in heart-transplanted children. Methods and results: Cardiac and endothelial chimerism was detected in endomyocardial biopsies of nine children (age 1 months-14 years) with sex-mismatched heart transplantation by fluorescence in situ hybridization. Time from transplantation to biopsy ranged from 1 month up to 10 years. The extent of repopulating cardiomyocytes was 2.39 ± 1.54% (range: 0-4.2%) and correlated significantly with the time from transplantation to biopsy sampling (r2 = 0.69, P = 0.006; n = 9). The calculated contribution of male cardiomyocytes in the female heart per year was 0.36 ± 0.09%. Consistent with the previous reports, the incorporation of vascular cells was higher compared with cardiomyocytes (14.4 ± 4.17%), but did not correlate in a time-dependent manner. Conclusion: Circulating cells contribute to cardiomyocytes and endothelial cells in children after heart transplantation. The incidence of repopulating cardiomyocytes continuously increases in a time-dependent manner (∼4% Y-chromosome+ cardiomyocytes/10 years) and resembles the cardiac regeneration activity observed in adults.

Original languageEnglish
Pages (from-to)1867-1872
Number of pages6
JournalEuropean heart journal
Volume29
Issue number15
DOIs
Publication statusPublished - Aug 1 2008

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Heart Transplantation
Cardiac Myocytes
Biopsy
Blood Vessels
Regeneration
Transplantation
Chromosomes, Human, Pair 10
Chimerism
Y Chromosome
Fluorescence In Situ Hybridization
Endothelial Cells
Incidence

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Rupp, S., Koyanagi, M., Iwasaki, M., Bauer, J., Von Gerlach, S., Schranz, D., ... Dimmeler, S. (2008). Characterization of long-term endogenous cardiac repair in children after heart transplantation. European heart journal, 29(15), 1867-1872. https://doi.org/10.1093/eurheartj/ehn223

Characterization of long-term endogenous cardiac repair in children after heart transplantation. / Rupp, Stefan; Koyanagi, Masamichi; Iwasaki, Masayoshi; Bauer, Jürgen; Von Gerlach, Susanne; Schranz, Dietmar; Zeiher, Andreas M.; Dimmeler, Stefanie.

In: European heart journal, Vol. 29, No. 15, 01.08.2008, p. 1867-1872.

Research output: Contribution to journalArticle

Rupp, S, Koyanagi, M, Iwasaki, M, Bauer, J, Von Gerlach, S, Schranz, D, Zeiher, AM & Dimmeler, S 2008, 'Characterization of long-term endogenous cardiac repair in children after heart transplantation', European heart journal, vol. 29, no. 15, pp. 1867-1872. https://doi.org/10.1093/eurheartj/ehn223
Rupp S, Koyanagi M, Iwasaki M, Bauer J, Von Gerlach S, Schranz D et al. Characterization of long-term endogenous cardiac repair in children after heart transplantation. European heart journal. 2008 Aug 1;29(15):1867-1872. https://doi.org/10.1093/eurheartj/ehn223
Rupp, Stefan ; Koyanagi, Masamichi ; Iwasaki, Masayoshi ; Bauer, Jürgen ; Von Gerlach, Susanne ; Schranz, Dietmar ; Zeiher, Andreas M. ; Dimmeler, Stefanie. / Characterization of long-term endogenous cardiac repair in children after heart transplantation. In: European heart journal. 2008 ; Vol. 29, No. 15. pp. 1867-1872.
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