Characterization of Lung Cancer by Amide Proton Transfer (APT) Imaging: An In-Vivo Study in an Orthotopic Mouse Model

Osamu Togao, Chase W. Kessinger, Gang Huang, Todd C. Soesbe, Koji Sagiyama, Ivan Dimitrov, A. Dean Sherry, Jinming Gao, Masaya Takahashi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Amide proton transfer (APT) imaging is one of the chemical exchange saturation transfer (CEST) imaging methods which images the exchange between protons of free tissue water and the amide groups (-NH) of endogenous mobile proteins and peptides. Previous work suggested the ability of APT imaging for characterization of the tumoral grade in the brain tumor. In this study, we tested the feasibility of in-vivo APT imaging of lung tumor and investigated whether the method could differentiate the tumoral types on orthotopic tumor xenografts from two malignant lung cancer cell lines. The results revealed that APT imaging is feasible to quantify lung tumors in the moving lung. The measured APT effect was higher in the tumor which exhibited more active proliferation than the other. The present study demonstrates that APT imaging has the potential to provide a characterization test to differentiate types or grade of lung cancer noninvasively, which may eventually reduce the need invasive needle biopsy or resection for lung cancer.

Original languageEnglish
Article numbere77019
JournalPloS one
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 15 2013

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Proton transfer
lung neoplasms
in vivo studies
amides
Amides
protons
Protons
Lung Neoplasms
animal models
image analysis
Imaging techniques
Tumors
Lung
neoplasms
Neoplasms
Biopsy
Needle Biopsy
resection
Heterografts
Brain Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Characterization of Lung Cancer by Amide Proton Transfer (APT) Imaging : An In-Vivo Study in an Orthotopic Mouse Model. / Togao, Osamu; Kessinger, Chase W.; Huang, Gang; Soesbe, Todd C.; Sagiyama, Koji; Dimitrov, Ivan; Sherry, A. Dean; Gao, Jinming; Takahashi, Masaya.

In: PloS one, Vol. 8, No. 10, e77019, 15.10.2013.

Research output: Contribution to journalArticle

Togao, Osamu ; Kessinger, Chase W. ; Huang, Gang ; Soesbe, Todd C. ; Sagiyama, Koji ; Dimitrov, Ivan ; Sherry, A. Dean ; Gao, Jinming ; Takahashi, Masaya. / Characterization of Lung Cancer by Amide Proton Transfer (APT) Imaging : An In-Vivo Study in an Orthotopic Mouse Model. In: PloS one. 2013 ; Vol. 8, No. 10.
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