Characterization of modification enzyme NukM and engineering of a novel thioether bridge in lantibiotic nukacin ISK-1

Kouki Shioya, Yoshitaka Harada, Jun Ichi Nagao, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The lantibiotic nukacin ISK-1 is an antimicrobial peptide containing unusual amino acids such as lanthionine and dehydrobutyrine. The nukacin ISK-1 prepeptide (NukA) undergoes posttranslational modifications, such as the dehydration and cyclization reactions required to form the unusual amino acids by the modification enzyme NukM. We have previously constructed a system for the introduction of unusual amino acids into NukA by coexpression of NukM in Escherichia coli. Using this system, we describe the substrate specificity of NukM by the coexpression of a series of NukA mutants. Our results revealed the following characteristics of NukM: (1) its dehydration activity is not coupled to its cyclization activity; (2) its dehydration activity is site-specific; (3) the length of the substrate is important for its dehydration activity. Furthermore, we succeeded in introducing a novel thioether bridge in NukA by replacing an unmodified Ser at position 27 with a Cys residue.

Original languageEnglish
Pages (from-to)891-899
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume86
Issue number3
DOIs
Publication statusPublished - Apr 1 2010

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Bacteriocins
Sulfides
Dehydration
Enzymes
Cyclization
Amino Acids
Post Translational Protein Processing
Substrate Specificity
Escherichia coli
Peptides
nukacin ISK-1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Characterization of modification enzyme NukM and engineering of a novel thioether bridge in lantibiotic nukacin ISK-1. / Shioya, Kouki; Harada, Yoshitaka; Nagao, Jun Ichi; Nakayama, Jiro; Sonomoto, Kenji.

In: Applied Microbiology and Biotechnology, Vol. 86, No. 3, 01.04.2010, p. 891-899.

Research output: Contribution to journalArticle

Shioya, Kouki ; Harada, Yoshitaka ; Nagao, Jun Ichi ; Nakayama, Jiro ; Sonomoto, Kenji. / Characterization of modification enzyme NukM and engineering of a novel thioether bridge in lantibiotic nukacin ISK-1. In: Applied Microbiology and Biotechnology. 2010 ; Vol. 86, No. 3. pp. 891-899.
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