Characterization of natriuretic peptide receptors in cultured cells

Shin Ichi Suga, Kazuwa Nakao, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Yoshihiro Ogawa, Hiroo Imura

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

To elucidate physiological and clinical implications of the natriuretic peptide family, the expression of receptors for natriuretic peptides has been examined in cultured cells (a rat pheochromocytoma cell line [PC12], bovine endothelial cells, rat aortic smooth muscle cells, human mesangial cells, and a porcine kidney epithelial cell line [LLC-PK1]) by Northern blot analysis and cyclic GMP production method for the ANP-A and ANP-B receptors and by Northern blot analysis and binding assay for the clearance receptor. The ANP-A receptor was predominantly expressed in PC12 cells, bovine endothelial cells, and LLC-PK, cells but was barely expressed in rat aortic smooth muscle cells and human mesangial cells. By contrast, the ANP-B receptor was the major subtype of the biologically active receptors in rat aortic smooth muscle cells and human mesangial cells. Only a small amount of the ANP-B receptor was detected in PC12 cells, bovine endothelial cells, and LLC-PK, cells. The clearance receptor was abundantly expressed in rat aortic smooth muscle cells and human mesangial cells and was also present in bovine endothelial cells, but it was undetectable in PC12 cells and LLC-PK1 cells. These results demonstrate that the expression of three natriuretic peptide receptors varies from cell to cell, which is relevant to cell- or tissue-specific action of the natriuretic peptide family.

Original languageEnglish
Pages (from-to)762-765
Number of pages4
JournalHypertension
Volume19
Issue number6
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

Fingerprint

Natriuretic Peptides
Peptide Receptors
Atrial Natriuretic Factor Receptors
Mesangial Cells
PC12 Cells
Cultured Cells
Smooth Muscle Myocytes
Endothelial Cells
Northern Blotting
LLC-PK1 Cells
Cyclic GMP
Atrial Natriuretic Factor
Swine
Epithelial Cells
Kidney
Cell Line

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Suga, S. I., Nakao, K., Mukoyama, M., Arai, H., Hosoda, K., Ogawa, Y., & Imura, H. (1992). Characterization of natriuretic peptide receptors in cultured cells. Hypertension, 19(6), 762-765. https://doi.org/10.1161/01.HYP.19.6.762

Characterization of natriuretic peptide receptors in cultured cells. / Suga, Shin Ichi; Nakao, Kazuwa; Mukoyama, Masashi; Arai, Hiroshi; Hosoda, Kiminori; Ogawa, Yoshihiro; Imura, Hiroo.

In: Hypertension, Vol. 19, No. 6, 01.01.1992, p. 762-765.

Research output: Contribution to journalArticle

Suga, SI, Nakao, K, Mukoyama, M, Arai, H, Hosoda, K, Ogawa, Y & Imura, H 1992, 'Characterization of natriuretic peptide receptors in cultured cells', Hypertension, vol. 19, no. 6, pp. 762-765. https://doi.org/10.1161/01.HYP.19.6.762
Suga, Shin Ichi ; Nakao, Kazuwa ; Mukoyama, Masashi ; Arai, Hiroshi ; Hosoda, Kiminori ; Ogawa, Yoshihiro ; Imura, Hiroo. / Characterization of natriuretic peptide receptors in cultured cells. In: Hypertension. 1992 ; Vol. 19, No. 6. pp. 762-765.
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