Characterization of NtChitIV, a class IV chitinase induced by β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102: Antagonistic effect of salicylic acid and methyl jasmonate on the induction of NtChitIV

Tomonori Shinya, Kazunari Hanai, Ivan Gális, Kaoru Suzuki, Ken Matsuoka, Hideaki Matsuoka, Mikako Saito

Research output: Contribution to journalArticle

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Abstract

Tobacco BY-2 class IV chitinases (TBC-1, TBC-3) were rapidly and transiently induced by the β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 (AaGlucan). The full-length cDNA and 5′-flanking region of a gene encoding class IV chitinases were isolated on the basis of the amino acid sequence of TBC-1. Sequence analysis indicated that NtChitIV encoded TBC-1, TBC-3, or both. Since purified TBC-1 and TBC-3 from BY-2 cells lack a chitin binding domain in the N-terminal region, these enzymes suggested to be derived from NtChitIV by post-translational proteolytic processing. The transcripts of NtChitIV accumulated rapidly within 1 h after treatment with AaGlucan. Accumulation was maximal 3 h after treatment. Reporter gene assays were used to analyze the promoter regions involved in the transcriptional control of NtChitIV, and these assays revealed that the 1.89-kb NtChitIV promoter was activated by AaGlucan but not by salicylic acid (SA) or methyl jasmonate (MeJA). The AaGlucan-induced transcriptional activation via 1.89-kb NtChitIV promoter was attenuated by pretreatment with SA or MeJA. These results suggest that NtChitIV expression is particularly induced by AaGlucan and that the AaGlucan-dependent signaling pathway is different from the SA- and MeJA-dependent signaling pathways.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number2
DOIs
Publication statusPublished - Feb 9 2007

Fingerprint

Alternaria
Chitinases
Salicylic Acid
Glucans
Assays
Gene encoding
Chitin
Tobacco
5' Flanking Region
Reporter Genes
Genetic Promoter Regions
Transcriptional Activation
Sequence Analysis
Amino Acid Sequence
Complementary DNA
Genes
Chemical activation
Amino Acids
Enzymes
Processing

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of NtChitIV, a class IV chitinase induced by β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 : Antagonistic effect of salicylic acid and methyl jasmonate on the induction of NtChitIV. / Shinya, Tomonori; Hanai, Kazunari; Gális, Ivan; Suzuki, Kaoru; Matsuoka, Ken; Matsuoka, Hideaki; Saito, Mikako.

In: Biochemical and Biophysical Research Communications, Vol. 353, No. 2, 09.02.2007, p. 311-317.

Research output: Contribution to journalArticle

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abstract = "Tobacco BY-2 class IV chitinases (TBC-1, TBC-3) were rapidly and transiently induced by the β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 (AaGlucan). The full-length cDNA and 5′-flanking region of a gene encoding class IV chitinases were isolated on the basis of the amino acid sequence of TBC-1. Sequence analysis indicated that NtChitIV encoded TBC-1, TBC-3, or both. Since purified TBC-1 and TBC-3 from BY-2 cells lack a chitin binding domain in the N-terminal region, these enzymes suggested to be derived from NtChitIV by post-translational proteolytic processing. The transcripts of NtChitIV accumulated rapidly within 1 h after treatment with AaGlucan. Accumulation was maximal 3 h after treatment. Reporter gene assays were used to analyze the promoter regions involved in the transcriptional control of NtChitIV, and these assays revealed that the 1.89-kb NtChitIV promoter was activated by AaGlucan but not by salicylic acid (SA) or methyl jasmonate (MeJA). The AaGlucan-induced transcriptional activation via 1.89-kb NtChitIV promoter was attenuated by pretreatment with SA or MeJA. These results suggest that NtChitIV expression is particularly induced by AaGlucan and that the AaGlucan-dependent signaling pathway is different from the SA- and MeJA-dependent signaling pathways.",
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AU - Shinya, Tomonori

AU - Hanai, Kazunari

AU - Gális, Ivan

AU - Suzuki, Kaoru

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