TY - JOUR
T1 - Characterization of NtChitIV, a class IV chitinase induced by β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102
T2 - Antagonistic effect of salicylic acid and methyl jasmonate on the induction of NtChitIV
AU - Shinya, Tomonori
AU - Hanai, Kazunari
AU - Gális, Ivan
AU - Suzuki, Kaoru
AU - Matsuoka, Ken
AU - Matsuoka, Hideaki
AU - Saito, Mikako
N1 - Funding Information:
We are grateful to Dr. Y. Ozeki, Dr. A. Yamada, and Dr. Y. Ito of the Tokyo University of Agriculture and Technology for their kind advice on the inverse PCR technique and BY-2 cell transformation. We also thank Dr. M. Yamaguchi of the RIKEN Plant Science Center for critical reading of the manuscript. This work was partly supported by a Grant-in-Aid for Scientific Research (Scientific Research Priority Area: Single-Cell Molecular Technology) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2007/2/9
Y1 - 2007/2/9
N2 - Tobacco BY-2 class IV chitinases (TBC-1, TBC-3) were rapidly and transiently induced by the β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 (AaGlucan). The full-length cDNA and 5′-flanking region of a gene encoding class IV chitinases were isolated on the basis of the amino acid sequence of TBC-1. Sequence analysis indicated that NtChitIV encoded TBC-1, TBC-3, or both. Since purified TBC-1 and TBC-3 from BY-2 cells lack a chitin binding domain in the N-terminal region, these enzymes suggested to be derived from NtChitIV by post-translational proteolytic processing. The transcripts of NtChitIV accumulated rapidly within 1 h after treatment with AaGlucan. Accumulation was maximal 3 h after treatment. Reporter gene assays were used to analyze the promoter regions involved in the transcriptional control of NtChitIV, and these assays revealed that the 1.89-kb NtChitIV promoter was activated by AaGlucan but not by salicylic acid (SA) or methyl jasmonate (MeJA). The AaGlucan-induced transcriptional activation via 1.89-kb NtChitIV promoter was attenuated by pretreatment with SA or MeJA. These results suggest that NtChitIV expression is particularly induced by AaGlucan and that the AaGlucan-dependent signaling pathway is different from the SA- and MeJA-dependent signaling pathways.
AB - Tobacco BY-2 class IV chitinases (TBC-1, TBC-3) were rapidly and transiently induced by the β-1,3-, 1,6-glucan elicitor from Alternaria alternata 102 (AaGlucan). The full-length cDNA and 5′-flanking region of a gene encoding class IV chitinases were isolated on the basis of the amino acid sequence of TBC-1. Sequence analysis indicated that NtChitIV encoded TBC-1, TBC-3, or both. Since purified TBC-1 and TBC-3 from BY-2 cells lack a chitin binding domain in the N-terminal region, these enzymes suggested to be derived from NtChitIV by post-translational proteolytic processing. The transcripts of NtChitIV accumulated rapidly within 1 h after treatment with AaGlucan. Accumulation was maximal 3 h after treatment. Reporter gene assays were used to analyze the promoter regions involved in the transcriptional control of NtChitIV, and these assays revealed that the 1.89-kb NtChitIV promoter was activated by AaGlucan but not by salicylic acid (SA) or methyl jasmonate (MeJA). The AaGlucan-induced transcriptional activation via 1.89-kb NtChitIV promoter was attenuated by pretreatment with SA or MeJA. These results suggest that NtChitIV expression is particularly induced by AaGlucan and that the AaGlucan-dependent signaling pathway is different from the SA- and MeJA-dependent signaling pathways.
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U2 - 10.1016/j.bbrc.2006.12.009
DO - 10.1016/j.bbrc.2006.12.009
M3 - Article
C2 - 17178105
AN - SCOPUS:33845864990
SN - 0006-291X
VL - 353
SP - 311
EP - 317
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -