Characterization of partially hydrolyzed OCP crystals deposited in a gelatin matrix as a scaffold for bone tissue engineering

Yushi Ezoe, Takahisa Anada, Hajime Yamazaki, Takuto Handa, Kazuhito Kobayashi, Tetsu Takahashi, Osamu Suzuki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present study was designed to investigate how hydrolysis of octacalcium phosphate (OCP) into hydroxyapatite is affected by the presence of gelatin (Gel) molecules and how osteoblastic cells respond to the resultant OCP hydrolyzate/Gel composites as the hydrolysis advances. OCP was prepared from a solution containing calcium and phosphate ions and Gel molecules, having a composition to produce a 40 wt% OCP as a final co-precipitate as the OCP/Gel. The precipitate was further incubated up to 40 h to advance the hydrolysis of OCP. These precipitates were processed to mold OCP/Gel sponges through lyophilization and dehydrothermal treatment. Chemical analysis, X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, and selected area electron diffraction revealed that the hydrolysis of OCP/Gel composite in hot water advanced in a time-dependent manner and faster than hydrolysis of OCP alone. The effect of Gel on the OCP hydrolysis was further examined in the presence of distinct concentrations of Gel molecules in hot water, showing that the Gel enhanced the hydrolysis as the concentration increased. Proliferation and differentiation of mouse bone marrow stromal ST-2 cells on the hydrolyzed OCP/Gel composites were compatible with Gel sponge alone after 21 days of culture, suggesting that these composites could be a candidate as a scaffold in bone tissue engineering.

Original languageEnglish
JournalJournal of Nanoparticle Research
Volume17
Issue number3
DOIs
Publication statusPublished - Jan 1 2015
Externally publishedYes

Fingerprint

Tissue Engineering
Scaffold
tissue engineering
gelatins
Gelatin
Scaffolds (biology)
Phosphate
Tissue engineering
Bone
bones
phosphates
Phosphates
Crystal
Crystals
Hydrolysis
matrices
hydrolysis
crystals
Composite
Precipitates

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Modelling and Simulation
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering

Cite this

Characterization of partially hydrolyzed OCP crystals deposited in a gelatin matrix as a scaffold for bone tissue engineering. / Ezoe, Yushi; Anada, Takahisa; Yamazaki, Hajime; Handa, Takuto; Kobayashi, Kazuhito; Takahashi, Tetsu; Suzuki, Osamu.

In: Journal of Nanoparticle Research, Vol. 17, No. 3, 01.01.2015.

Research output: Contribution to journalArticle

Ezoe, Yushi ; Anada, Takahisa ; Yamazaki, Hajime ; Handa, Takuto ; Kobayashi, Kazuhito ; Takahashi, Tetsu ; Suzuki, Osamu. / Characterization of partially hydrolyzed OCP crystals deposited in a gelatin matrix as a scaffold for bone tissue engineering. In: Journal of Nanoparticle Research. 2015 ; Vol. 17, No. 3.
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