Characterization of polyelectrolyte-protein multilayer films by atomic force microscopy, scanning electron microscopy, and fourier transform infrared reflection-absorption spectroscopy

Frank Caruso, D. Neil Furlong, Katsuhiko Ariga, Izumi Ichinose, Toyoki Kunitake

Research output: Contribution to journalArticle

311 Citations (Scopus)

Abstract

Protein-containing polyelectrolyte multilayer films of poly(styrenesulfonate) and poly(allylamine hydrochloride), fabricated by the sequential adsorption of polyelectrolyte and anti-immunoglobulin G (anti-IgG) on solid substrates, have been characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), and Fourier transform infrared reflection-absorption spectroscopy (FTIRRAS). Visualization of the film structure on the nanometer scale, by AFM and SEM, showed that either layered or disordered films were formed depending on the number of polyelectrolyte layers separating each protein layer. For films where each anti-IgG layer was separated by one polyelectrolyte layer, an open, disordered film structure was observed and significant protein aggregation occurred. In contrast, for films in which the anti-IgG layers were separated by five polyelectrolyte layers, a layered structure with uniform protein layers was formed. Film thicknesses determined by SEM measurements were consistent with those calculated from quartz crystal microbalance measurements. FTIR-RAS confirmed the presence of anti-IgG in the multilayer films, with the amide I and II bands due to anti-IgG clearly visible in the spectra, and provided direct evidence that anti-IgG was not denatured. Both types of films fabricated are interesting for biosensing applications: the first provides ordered, functional protein layers within a polyelectrolyte matrix for sensing investigations, and the second serves as a useful functional film for applications where an increased binding capacity of the film is sought.

Original languageEnglish
Pages (from-to)4559-4565
Number of pages7
JournalLangmuir
Volume14
Issue number16
Publication statusPublished - Aug 4 1998

Fingerprint

infrared reflection
Multilayer films
Polyelectrolytes
Absorption spectroscopy
Atomic force microscopy
Fourier transforms
absorption spectroscopy
atomic force microscopy
Infrared radiation
proteins
Proteins
Scanning electron microscopy
scanning electron microscopy
Immunoglobulin G
Quartz crystal microbalances
Amides
Film thickness
hydrochlorides
quartz crystals
Agglomeration

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Characterization of polyelectrolyte-protein multilayer films by atomic force microscopy, scanning electron microscopy, and fourier transform infrared reflection-absorption spectroscopy. / Caruso, Frank; Furlong, D. Neil; Ariga, Katsuhiko; Ichinose, Izumi; Kunitake, Toyoki.

In: Langmuir, Vol. 14, No. 16, 04.08.1998, p. 4559-4565.

Research output: Contribution to journalArticle

Caruso, Frank ; Furlong, D. Neil ; Ariga, Katsuhiko ; Ichinose, Izumi ; Kunitake, Toyoki. / Characterization of polyelectrolyte-protein multilayer films by atomic force microscopy, scanning electron microscopy, and fourier transform infrared reflection-absorption spectroscopy. In: Langmuir. 1998 ; Vol. 14, No. 16. pp. 4559-4565.
@article{d66373fdaa59403d88a1c1b76972f88c,
title = "Characterization of polyelectrolyte-protein multilayer films by atomic force microscopy, scanning electron microscopy, and fourier transform infrared reflection-absorption spectroscopy",
abstract = "Protein-containing polyelectrolyte multilayer films of poly(styrenesulfonate) and poly(allylamine hydrochloride), fabricated by the sequential adsorption of polyelectrolyte and anti-immunoglobulin G (anti-IgG) on solid substrates, have been characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), and Fourier transform infrared reflection-absorption spectroscopy (FTIRRAS). Visualization of the film structure on the nanometer scale, by AFM and SEM, showed that either layered or disordered films were formed depending on the number of polyelectrolyte layers separating each protein layer. For films where each anti-IgG layer was separated by one polyelectrolyte layer, an open, disordered film structure was observed and significant protein aggregation occurred. In contrast, for films in which the anti-IgG layers were separated by five polyelectrolyte layers, a layered structure with uniform protein layers was formed. Film thicknesses determined by SEM measurements were consistent with those calculated from quartz crystal microbalance measurements. FTIR-RAS confirmed the presence of anti-IgG in the multilayer films, with the amide I and II bands due to anti-IgG clearly visible in the spectra, and provided direct evidence that anti-IgG was not denatured. Both types of films fabricated are interesting for biosensing applications: the first provides ordered, functional protein layers within a polyelectrolyte matrix for sensing investigations, and the second serves as a useful functional film for applications where an increased binding capacity of the film is sought.",
author = "Frank Caruso and Furlong, {D. Neil} and Katsuhiko Ariga and Izumi Ichinose and Toyoki Kunitake",
year = "1998",
month = "8",
day = "4",
language = "English",
volume = "14",
pages = "4559--4565",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "16",

}

TY - JOUR

T1 - Characterization of polyelectrolyte-protein multilayer films by atomic force microscopy, scanning electron microscopy, and fourier transform infrared reflection-absorption spectroscopy

AU - Caruso, Frank

AU - Furlong, D. Neil

AU - Ariga, Katsuhiko

AU - Ichinose, Izumi

AU - Kunitake, Toyoki

PY - 1998/8/4

Y1 - 1998/8/4

N2 - Protein-containing polyelectrolyte multilayer films of poly(styrenesulfonate) and poly(allylamine hydrochloride), fabricated by the sequential adsorption of polyelectrolyte and anti-immunoglobulin G (anti-IgG) on solid substrates, have been characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), and Fourier transform infrared reflection-absorption spectroscopy (FTIRRAS). Visualization of the film structure on the nanometer scale, by AFM and SEM, showed that either layered or disordered films were formed depending on the number of polyelectrolyte layers separating each protein layer. For films where each anti-IgG layer was separated by one polyelectrolyte layer, an open, disordered film structure was observed and significant protein aggregation occurred. In contrast, for films in which the anti-IgG layers were separated by five polyelectrolyte layers, a layered structure with uniform protein layers was formed. Film thicknesses determined by SEM measurements were consistent with those calculated from quartz crystal microbalance measurements. FTIR-RAS confirmed the presence of anti-IgG in the multilayer films, with the amide I and II bands due to anti-IgG clearly visible in the spectra, and provided direct evidence that anti-IgG was not denatured. Both types of films fabricated are interesting for biosensing applications: the first provides ordered, functional protein layers within a polyelectrolyte matrix for sensing investigations, and the second serves as a useful functional film for applications where an increased binding capacity of the film is sought.

AB - Protein-containing polyelectrolyte multilayer films of poly(styrenesulfonate) and poly(allylamine hydrochloride), fabricated by the sequential adsorption of polyelectrolyte and anti-immunoglobulin G (anti-IgG) on solid substrates, have been characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), and Fourier transform infrared reflection-absorption spectroscopy (FTIRRAS). Visualization of the film structure on the nanometer scale, by AFM and SEM, showed that either layered or disordered films were formed depending on the number of polyelectrolyte layers separating each protein layer. For films where each anti-IgG layer was separated by one polyelectrolyte layer, an open, disordered film structure was observed and significant protein aggregation occurred. In contrast, for films in which the anti-IgG layers were separated by five polyelectrolyte layers, a layered structure with uniform protein layers was formed. Film thicknesses determined by SEM measurements were consistent with those calculated from quartz crystal microbalance measurements. FTIR-RAS confirmed the presence of anti-IgG in the multilayer films, with the amide I and II bands due to anti-IgG clearly visible in the spectra, and provided direct evidence that anti-IgG was not denatured. Both types of films fabricated are interesting for biosensing applications: the first provides ordered, functional protein layers within a polyelectrolyte matrix for sensing investigations, and the second serves as a useful functional film for applications where an increased binding capacity of the film is sought.

UR - http://www.scopus.com/inward/record.url?scp=0032482895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032482895&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0032482895

VL - 14

SP - 4559

EP - 4565

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 16

ER -