Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation

Toshihiro Watanabe, Seiji Misawa, Syuntaro Hiradate, Mitsuru Osaki

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

• Plant roots exude viscous polysaccharides, called mucilage. One of the suggested roles of mucilage is immobilization of toxic metal cations, including aluminum (Al), in the rhizosphere. • Mucilage exuded from roots of Melastoma malabathricum (Al accumulator) was characterized in comparison with that of Zea mays (maize; Al nonaccumulator). • Removal of mucilage significantly reduced Al accumulation in M. malabathricum. The cation adsorption affinity of M. malabathricum mucilage was higher for Al and lanthanum (La) than for barium (Ba), whereas that of maize mucilage was in the order Ba > La > Al. A 27Al nuclear magnetic resonance (NMR) spectrum of the Al-adsorbed mucilage and bioassay with alfalfa seedlings indicated that the concentrated Al in the mucilage of M. malabathricum, unlike that of maize, bound very weakly to cation exchange sites of mucilage. • The higher charge density in M. malabathricum mucilage, derived from unmethylated uronic acid, is inferred to be related to preferential adsorption of trivalent cation. Not only a higher degree of methylation in the uronic acid (glucuronic acid) but also H+ release from roots to the mucilage appears to be responsible for the loose binding of Al in M. malabathricum mucilage. These characteristics of mucilage may help Al hyperaccumulation in M. malabathricum.

Original languageEnglish
Pages (from-to)581-589
Number of pages9
JournalNew Phytologist
Volume178
Issue number3
DOIs
Publication statusPublished - May 1 2008

Fingerprint

Melastoma malabathricum
mucilages
Aluminum
aluminum
Zea mays
Cations
Uronic Acids
Lanthanum
Barium
lanthanum
cations
barium
Adsorption
uronic acids
corn
adsorption
Glucuronic Acid
Rhizosphere
Plant Roots
Methylation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation. / Watanabe, Toshihiro; Misawa, Seiji; Hiradate, Syuntaro; Osaki, Mitsuru.

In: New Phytologist, Vol. 178, No. 3, 01.05.2008, p. 581-589.

Research output: Contribution to journalArticle

@article{663d7a915d3447f6bdb5de758e191f62,
title = "Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation",
abstract = "• Plant roots exude viscous polysaccharides, called mucilage. One of the suggested roles of mucilage is immobilization of toxic metal cations, including aluminum (Al), in the rhizosphere. • Mucilage exuded from roots of Melastoma malabathricum (Al accumulator) was characterized in comparison with that of Zea mays (maize; Al nonaccumulator). • Removal of mucilage significantly reduced Al accumulation in M. malabathricum. The cation adsorption affinity of M. malabathricum mucilage was higher for Al and lanthanum (La) than for barium (Ba), whereas that of maize mucilage was in the order Ba > La > Al. A 27Al nuclear magnetic resonance (NMR) spectrum of the Al-adsorbed mucilage and bioassay with alfalfa seedlings indicated that the concentrated Al in the mucilage of M. malabathricum, unlike that of maize, bound very weakly to cation exchange sites of mucilage. • The higher charge density in M. malabathricum mucilage, derived from unmethylated uronic acid, is inferred to be related to preferential adsorption of trivalent cation. Not only a higher degree of methylation in the uronic acid (glucuronic acid) but also H+ release from roots to the mucilage appears to be responsible for the loose binding of Al in M. malabathricum mucilage. These characteristics of mucilage may help Al hyperaccumulation in M. malabathricum.",
author = "Toshihiro Watanabe and Seiji Misawa and Syuntaro Hiradate and Mitsuru Osaki",
year = "2008",
month = "5",
day = "1",
doi = "10.1111/j.1469-8137.2008.02397.x",
language = "English",
volume = "178",
pages = "581--589",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Characterization of root mucilage from Melastoma malabathricum, with emphasis on its roles in aluminum accumulation

AU - Watanabe, Toshihiro

AU - Misawa, Seiji

AU - Hiradate, Syuntaro

AU - Osaki, Mitsuru

PY - 2008/5/1

Y1 - 2008/5/1

N2 - • Plant roots exude viscous polysaccharides, called mucilage. One of the suggested roles of mucilage is immobilization of toxic metal cations, including aluminum (Al), in the rhizosphere. • Mucilage exuded from roots of Melastoma malabathricum (Al accumulator) was characterized in comparison with that of Zea mays (maize; Al nonaccumulator). • Removal of mucilage significantly reduced Al accumulation in M. malabathricum. The cation adsorption affinity of M. malabathricum mucilage was higher for Al and lanthanum (La) than for barium (Ba), whereas that of maize mucilage was in the order Ba > La > Al. A 27Al nuclear magnetic resonance (NMR) spectrum of the Al-adsorbed mucilage and bioassay with alfalfa seedlings indicated that the concentrated Al in the mucilage of M. malabathricum, unlike that of maize, bound very weakly to cation exchange sites of mucilage. • The higher charge density in M. malabathricum mucilage, derived from unmethylated uronic acid, is inferred to be related to preferential adsorption of trivalent cation. Not only a higher degree of methylation in the uronic acid (glucuronic acid) but also H+ release from roots to the mucilage appears to be responsible for the loose binding of Al in M. malabathricum mucilage. These characteristics of mucilage may help Al hyperaccumulation in M. malabathricum.

AB - • Plant roots exude viscous polysaccharides, called mucilage. One of the suggested roles of mucilage is immobilization of toxic metal cations, including aluminum (Al), in the rhizosphere. • Mucilage exuded from roots of Melastoma malabathricum (Al accumulator) was characterized in comparison with that of Zea mays (maize; Al nonaccumulator). • Removal of mucilage significantly reduced Al accumulation in M. malabathricum. The cation adsorption affinity of M. malabathricum mucilage was higher for Al and lanthanum (La) than for barium (Ba), whereas that of maize mucilage was in the order Ba > La > Al. A 27Al nuclear magnetic resonance (NMR) spectrum of the Al-adsorbed mucilage and bioassay with alfalfa seedlings indicated that the concentrated Al in the mucilage of M. malabathricum, unlike that of maize, bound very weakly to cation exchange sites of mucilage. • The higher charge density in M. malabathricum mucilage, derived from unmethylated uronic acid, is inferred to be related to preferential adsorption of trivalent cation. Not only a higher degree of methylation in the uronic acid (glucuronic acid) but also H+ release from roots to the mucilage appears to be responsible for the loose binding of Al in M. malabathricum mucilage. These characteristics of mucilage may help Al hyperaccumulation in M. malabathricum.

UR - http://www.scopus.com/inward/record.url?scp=42249098424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42249098424&partnerID=8YFLogxK

U2 - 10.1111/j.1469-8137.2008.02397.x

DO - 10.1111/j.1469-8137.2008.02397.x

M3 - Article

VL - 178

SP - 581

EP - 589

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 3

ER -