Characterization of side-population cells in human normal endometrium

Kiyoko Kato, Momoko Yoshimoto, Keiji Kato, Sawako Adachi, Asako Yamayoshi, Takahiro Arima, Kazuo Asanoma, Satoru Kyo, Tatsutoshi Nakahata, Norio Wake

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Background: It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties. Method: Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS. Results: SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9-CD13- fraction. These SP cells showed long-term repopulating properties and produced gland (CD9+)- and stroma (CD13+)-like cells. CD9-CD13- cells isolated from the endometrium also generated gland- or stroma-like cells. Conclusions: SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells.

Original languageEnglish
Pages (from-to)1214-1223
Number of pages10
JournalHuman Reproduction
Volume22
Issue number5
DOIs
Publication statusPublished - May 2007

Fingerprint

Side-Population Cells
Endometrium
Stem Cells
Fluorescence
Myoma
Adult Stem Cells
Verapamil
Hysterectomy
Digestion
Coloring Agents
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Kato, K., Yoshimoto, M., Kato, K., Adachi, S., Yamayoshi, A., Arima, T., ... Wake, N. (2007). Characterization of side-population cells in human normal endometrium. Human Reproduction, 22(5), 1214-1223. https://doi.org/10.1093/humrep/del514

Characterization of side-population cells in human normal endometrium. / Kato, Kiyoko; Yoshimoto, Momoko; Kato, Keiji; Adachi, Sawako; Yamayoshi, Asako; Arima, Takahiro; Asanoma, Kazuo; Kyo, Satoru; Nakahata, Tatsutoshi; Wake, Norio.

In: Human Reproduction, Vol. 22, No. 5, 05.2007, p. 1214-1223.

Research output: Contribution to journalArticle

Kato, K, Yoshimoto, M, Kato, K, Adachi, S, Yamayoshi, A, Arima, T, Asanoma, K, Kyo, S, Nakahata, T & Wake, N 2007, 'Characterization of side-population cells in human normal endometrium', Human Reproduction, vol. 22, no. 5, pp. 1214-1223. https://doi.org/10.1093/humrep/del514
Kato K, Yoshimoto M, Kato K, Adachi S, Yamayoshi A, Arima T et al. Characterization of side-population cells in human normal endometrium. Human Reproduction. 2007 May;22(5):1214-1223. https://doi.org/10.1093/humrep/del514
Kato, Kiyoko ; Yoshimoto, Momoko ; Kato, Keiji ; Adachi, Sawako ; Yamayoshi, Asako ; Arima, Takahiro ; Asanoma, Kazuo ; Kyo, Satoru ; Nakahata, Tatsutoshi ; Wake, Norio. / Characterization of side-population cells in human normal endometrium. In: Human Reproduction. 2007 ; Vol. 22, No. 5. pp. 1214-1223.
@article{6d414c8cb3544dc98c7b516f514399e7,
title = "Characterization of side-population cells in human normal endometrium",
abstract = "Background: It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties. Method: Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS. Results: SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9-CD13- fraction. These SP cells showed long-term repopulating properties and produced gland (CD9+)- and stroma (CD13+)-like cells. CD9-CD13- cells isolated from the endometrium also generated gland- or stroma-like cells. Conclusions: SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells.",
author = "Kiyoko Kato and Momoko Yoshimoto and Keiji Kato and Sawako Adachi and Asako Yamayoshi and Takahiro Arima and Kazuo Asanoma and Satoru Kyo and Tatsutoshi Nakahata and Norio Wake",
year = "2007",
month = "5",
doi = "10.1093/humrep/del514",
language = "English",
volume = "22",
pages = "1214--1223",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "5",

}

TY - JOUR

T1 - Characterization of side-population cells in human normal endometrium

AU - Kato, Kiyoko

AU - Yoshimoto, Momoko

AU - Kato, Keiji

AU - Adachi, Sawako

AU - Yamayoshi, Asako

AU - Arima, Takahiro

AU - Asanoma, Kazuo

AU - Kyo, Satoru

AU - Nakahata, Tatsutoshi

AU - Wake, Norio

PY - 2007/5

Y1 - 2007/5

N2 - Background: It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties. Method: Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS. Results: SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9-CD13- fraction. These SP cells showed long-term repopulating properties and produced gland (CD9+)- and stroma (CD13+)-like cells. CD9-CD13- cells isolated from the endometrium also generated gland- or stroma-like cells. Conclusions: SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells.

AB - Background: It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties. Method: Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS. Results: SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9-CD13- fraction. These SP cells showed long-term repopulating properties and produced gland (CD9+)- and stroma (CD13+)-like cells. CD9-CD13- cells isolated from the endometrium also generated gland- or stroma-like cells. Conclusions: SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells.

UR - http://www.scopus.com/inward/record.url?scp=34447098515&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447098515&partnerID=8YFLogxK

U2 - 10.1093/humrep/del514

DO - 10.1093/humrep/del514

M3 - Article

C2 - 17283036

AN - SCOPUS:34447098515

VL - 22

SP - 1214

EP - 1223

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 5

ER -