Characterization of T cell epitopes restricted by HLA-DP9 in streptococcal M12 protein

R. P. Dong, N. Kamikawaji, N. Toida, Y. Fujita, A. Kimura, Takehiko Sasazuki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Interaction of the HLA-DP9 (DPA1*0201/DPB1*0901) molecule and M protein of serotype 12 (SS95/12) streptococci, a main component of the streptococcal cell wall Ag, has been investigated to decipher peptide-binding capacity and T cell activation in the context of the HLA-DP molecule. Seven antigenic peptides (amino acids 19-25) restricted by the HLA-DP9 molecule were identified in M12 protein, using M12 protein- or peptide-specific T cell lines from naturally exposed individuals. The binding affinity of each peptide to the HLA-DP9 molecule was measured by fluorescence intensity of biotinylated peptides bound to L cell transfectants expressing HLA-DP9, followed by treatment with avidin-fluorescence. Binding of biotinylated peptides to the HLA-DP9 molecule was inhibited by an excess amount of corresponding nonbiotinylated peptides and other nonbiotinylated peptides, indicating that the peptides were bound to the HLA-DP9 molecule at a single binding site. Seven synthetic peptides containing the T cell epitopes restricted by the HLA-DP9 molecule had high binding affinity to the HLA-DP9 molecule. Comparison of the amino acid sequences of truncated analogues that could bind to the HLA-DP9 molecule and/or activate T cells suggested an HLA- DP9-specific binding motif, composed of a positively charged residue (R or K) at position 1, a hydrophobic residue (A, G, or L) at position 6, and another hydrophobic residue (L or V) at position 9. Analysis of single amino acid- substituted analogues suggested that the positively charged amino acid in the motif served as a key anchor residue for binding to the HLA-DP9 molecule, which differs from the binding motif to the HLA-DR molecules.

Original languageEnglish
Pages (from-to)4536-4545
Number of pages10
JournalJournal of Immunology
Volume154
Issue number9
Publication statusPublished - Jan 1 1995

Fingerprint

T-Lymphocyte Epitopes
Peptides
Proteins
Peptide T
T-Lymphocytes
Fluorescence
HLA-DP Antigens
Amino Acids
Amino Acid Motifs
Avidin
HLA-DR Antigens
Streptococcus
Cell Wall
Amino Acid Sequence
Binding Sites
Cell Line

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Dong, R. P., Kamikawaji, N., Toida, N., Fujita, Y., Kimura, A., & Sasazuki, T. (1995). Characterization of T cell epitopes restricted by HLA-DP9 in streptococcal M12 protein. Journal of Immunology, 154(9), 4536-4545.

Characterization of T cell epitopes restricted by HLA-DP9 in streptococcal M12 protein. / Dong, R. P.; Kamikawaji, N.; Toida, N.; Fujita, Y.; Kimura, A.; Sasazuki, Takehiko.

In: Journal of Immunology, Vol. 154, No. 9, 01.01.1995, p. 4536-4545.

Research output: Contribution to journalArticle

Dong, RP, Kamikawaji, N, Toida, N, Fujita, Y, Kimura, A & Sasazuki, T 1995, 'Characterization of T cell epitopes restricted by HLA-DP9 in streptococcal M12 protein', Journal of Immunology, vol. 154, no. 9, pp. 4536-4545.
Dong RP, Kamikawaji N, Toida N, Fujita Y, Kimura A, Sasazuki T. Characterization of T cell epitopes restricted by HLA-DP9 in streptococcal M12 protein. Journal of Immunology. 1995 Jan 1;154(9):4536-4545.
Dong, R. P. ; Kamikawaji, N. ; Toida, N. ; Fujita, Y. ; Kimura, A. ; Sasazuki, Takehiko. / Characterization of T cell epitopes restricted by HLA-DP9 in streptococcal M12 protein. In: Journal of Immunology. 1995 ; Vol. 154, No. 9. pp. 4536-4545.
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