Characterization of T-cell receptor γδ T cells appearing at the early phase of murine Listeria monocytogenes infection

G. Matsuzaki, K. Hiromatsu, Y. Yoshikai, K. Muramori, K. Nomoto

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    It has been shown that T-cell receptor (TcR) γδ+ CD4- CD8- T cells increase in number and have an important role in early protection in murine Listeria monocytogenes infection. In this report, to characterize further the phenotype of the γδ T cells in listeriosis, we analysed V region gene usage and in vitro antigen recognition of the TcR γδ T cells in the peritoneal cavity of mice at the early phase after i.p. infection with a sublethal dose of L. monocytogenes. The γδ T cells predominantly expressed Vδ6 which has been reported to be expressed by TcR γδ-bearing foetal thymocyte hybridomas specific to mycobacterial and self heat-shock protein (hsp) 60. These early appearing CD3+ CD4- CD8- T cells in Listeria-infected mice, which were reported to be TcR γδ T cells, increased in proportion and in size by in vitro stimulation with recombinant hsp 60 from Mycobacterium bovis and purified protein derivative from M. tuberculosis but not by stimulation with heat-killed L. monocytogenes. A 65,000 MW molecule was detected in the lysate of viable L. monocytogenes but not in the lysate of heat-killed L. monocytogenes by a monoclonal antibody (mAb) raised against mycobacterial hsp 60. These results suggest that the Vδ6-bearing peripheral γδ T cells are activated by recognizing listerial hsp 60 expressed by viable L. monocytogenes. The hsp 60-reactive Vδ6-bearing T cells may have an important role in protection against L. monocytogenes and other parasites that express hsp 60 at high level.

    Original languageEnglish
    Pages (from-to)22-27
    Number of pages6
    JournalImmunology
    Volume78
    Issue number1
    Publication statusPublished - 1993

    Fingerprint

    Listeriosis
    Listeria monocytogenes
    T-Cell Antigen Receptor
    Chaperonin 60
    T-Lymphocytes
    Hot Temperature
    Listeria
    Peritoneal Cavity
    Hybridomas
    Thymocytes
    Mycobacterium bovis
    Recombinant Proteins
    Parasites
    Tuberculosis
    Monoclonal Antibodies
    Phenotype
    Infection

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Matsuzaki, G., Hiromatsu, K., Yoshikai, Y., Muramori, K., & Nomoto, K. (1993). Characterization of T-cell receptor γδ T cells appearing at the early phase of murine Listeria monocytogenes infection. Immunology, 78(1), 22-27.

    Characterization of T-cell receptor γδ T cells appearing at the early phase of murine Listeria monocytogenes infection. / Matsuzaki, G.; Hiromatsu, K.; Yoshikai, Y.; Muramori, K.; Nomoto, K.

    In: Immunology, Vol. 78, No. 1, 1993, p. 22-27.

    Research output: Contribution to journalArticle

    Matsuzaki, G, Hiromatsu, K, Yoshikai, Y, Muramori, K & Nomoto, K 1993, 'Characterization of T-cell receptor γδ T cells appearing at the early phase of murine Listeria monocytogenes infection', Immunology, vol. 78, no. 1, pp. 22-27.
    Matsuzaki, G. ; Hiromatsu, K. ; Yoshikai, Y. ; Muramori, K. ; Nomoto, K. / Characterization of T-cell receptor γδ T cells appearing at the early phase of murine Listeria monocytogenes infection. In: Immunology. 1993 ; Vol. 78, No. 1. pp. 22-27.
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