Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1)

Shinji Fukuda, Yuki Yamasaki, Toru Iwaki, Hiroaki Kawasaki, Shizuka Akieda, Naruhiko Fukuchi, Tomoko Tahira, Kenshi Hayashi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The c-myc intron binding protein 1 (M1BP1) is a gigantic zinc finger protein comprising 2,437 amino acids and belonging to the MHC binding protein (MBP) family. MIBP1 is suggested to be a transcription factor involved in various biological functions. We show here that MIBP1 represses c-myc transcription from the major promoter, P2. Screening by the yeast two-hybrid system revealed that the MIBP1 protein interacts with the Ski-interacting protein (SKIP). In vitro pull-down assays and in vivo co-immunoprecipitation experiments confirmed this interaction. The acidic region of MIBP1 was found to be the site of interaction with the N-terminal half of SKIP. In situ hybridization analysis using developing rat embryos revealed that the MIBP1 mRNA is highly expressed in post-mitotic neurons, but the expression in immature neuroepithelium is low. The expression of MIBP1 in adult rat brain is also predominantly in neuronal cells, indicating that MIBP1 is involved in the physiology of mature neuronal cells.

Original languageEnglish
Pages (from-to)349-357
Number of pages9
JournalJournal of biochemistry
Volume131
Issue number3
DOIs
Publication statusPublished - Mar 2002

Fingerprint

Introns
Carrier Proteins
Transcription Factors
Rats
Proteins
Two-Hybrid System Techniques
Zinc Fingers
Physiology
Transcription
Hybrid systems
Immunoprecipitation
Yeast
Neurons
In Situ Hybridization
Zinc
Assays
Brain
Screening
Embryonic Structures
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1). / Fukuda, Shinji; Yamasaki, Yuki; Iwaki, Toru; Kawasaki, Hiroaki; Akieda, Shizuka; Fukuchi, Naruhiko; Tahira, Tomoko; Hayashi, Kenshi.

In: Journal of biochemistry, Vol. 131, No. 3, 03.2002, p. 349-357.

Research output: Contribution to journalArticle

Fukuda, Shinji ; Yamasaki, Yuki ; Iwaki, Toru ; Kawasaki, Hiroaki ; Akieda, Shizuka ; Fukuchi, Naruhiko ; Tahira, Tomoko ; Hayashi, Kenshi. / Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1). In: Journal of biochemistry. 2002 ; Vol. 131, No. 3. pp. 349-357.
@article{e8a8d2ce52354bed9275e5044e3ae0ac,
title = "Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1)",
abstract = "The c-myc intron binding protein 1 (M1BP1) is a gigantic zinc finger protein comprising 2,437 amino acids and belonging to the MHC binding protein (MBP) family. MIBP1 is suggested to be a transcription factor involved in various biological functions. We show here that MIBP1 represses c-myc transcription from the major promoter, P2. Screening by the yeast two-hybrid system revealed that the MIBP1 protein interacts with the Ski-interacting protein (SKIP). In vitro pull-down assays and in vivo co-immunoprecipitation experiments confirmed this interaction. The acidic region of MIBP1 was found to be the site of interaction with the N-terminal half of SKIP. In situ hybridization analysis using developing rat embryos revealed that the MIBP1 mRNA is highly expressed in post-mitotic neurons, but the expression in immature neuroepithelium is low. The expression of MIBP1 in adult rat brain is also predominantly in neuronal cells, indicating that MIBP1 is involved in the physiology of mature neuronal cells.",
author = "Shinji Fukuda and Yuki Yamasaki and Toru Iwaki and Hiroaki Kawasaki and Shizuka Akieda and Naruhiko Fukuchi and Tomoko Tahira and Kenshi Hayashi",
year = "2002",
month = "3",
doi = "10.1093/oxfordjournals.jbchem.a003109",
language = "English",
volume = "131",
pages = "349--357",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Characterization of the biological functions of a transcription factor, c-myc intron binding protein 1 (MIBP1)

AU - Fukuda, Shinji

AU - Yamasaki, Yuki

AU - Iwaki, Toru

AU - Kawasaki, Hiroaki

AU - Akieda, Shizuka

AU - Fukuchi, Naruhiko

AU - Tahira, Tomoko

AU - Hayashi, Kenshi

PY - 2002/3

Y1 - 2002/3

N2 - The c-myc intron binding protein 1 (M1BP1) is a gigantic zinc finger protein comprising 2,437 amino acids and belonging to the MHC binding protein (MBP) family. MIBP1 is suggested to be a transcription factor involved in various biological functions. We show here that MIBP1 represses c-myc transcription from the major promoter, P2. Screening by the yeast two-hybrid system revealed that the MIBP1 protein interacts with the Ski-interacting protein (SKIP). In vitro pull-down assays and in vivo co-immunoprecipitation experiments confirmed this interaction. The acidic region of MIBP1 was found to be the site of interaction with the N-terminal half of SKIP. In situ hybridization analysis using developing rat embryos revealed that the MIBP1 mRNA is highly expressed in post-mitotic neurons, but the expression in immature neuroepithelium is low. The expression of MIBP1 in adult rat brain is also predominantly in neuronal cells, indicating that MIBP1 is involved in the physiology of mature neuronal cells.

AB - The c-myc intron binding protein 1 (M1BP1) is a gigantic zinc finger protein comprising 2,437 amino acids and belonging to the MHC binding protein (MBP) family. MIBP1 is suggested to be a transcription factor involved in various biological functions. We show here that MIBP1 represses c-myc transcription from the major promoter, P2. Screening by the yeast two-hybrid system revealed that the MIBP1 protein interacts with the Ski-interacting protein (SKIP). In vitro pull-down assays and in vivo co-immunoprecipitation experiments confirmed this interaction. The acidic region of MIBP1 was found to be the site of interaction with the N-terminal half of SKIP. In situ hybridization analysis using developing rat embryos revealed that the MIBP1 mRNA is highly expressed in post-mitotic neurons, but the expression in immature neuroepithelium is low. The expression of MIBP1 in adult rat brain is also predominantly in neuronal cells, indicating that MIBP1 is involved in the physiology of mature neuronal cells.

UR - http://www.scopus.com/inward/record.url?scp=0036221001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036221001&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a003109

DO - 10.1093/oxfordjournals.jbchem.a003109

M3 - Article

C2 - 11872163

AN - SCOPUS:0036221001

VL - 131

SP - 349

EP - 357

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 3

ER -