TY - GEN
T1 - Characterization of the cellular biomechanlcal responses caused on microprocessed substrates
T2 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
AU - Kidoaki, Satoru
AU - Matsuda, Takehisa
PY - 2006
Y1 - 2006
N2 - To investigate the shape-dependent cell mechanics and ECM dependent control of motility, cell elasticity, structural features of the actin cytoskeleton (CSK) and motility responses were characterized on well-designed microprocessed substrates with micropatterned cell adhesive regions and microelastic gradient. Cell elasticity and atin CSK features of shape-engineered fibroblasts and vascular endothelial cells (round and spindle shaped) cultured on photolithographically microprocessed surfaces were characterized employing the cellular microindentation test and fluorescence observation of actin CSK by the combination of atomic force microscopy (AFM) and fluorescence microscopy (FM). Condition of directional cell movement towards stiffer region, so called mechanotaxis, was studied on the photolithographically-microprocessed microelastic gradient gel (MEG gel). The inter relationships between cell elasticity, the structural features of actin CSK and engineered cell shape were analyzed as compared with control cells cultured on non-processed surfaces (termed naturally extended cells). The results showed that the elasticity of regionally restricted adhesion-surface-induced shape-engineered cells, particularly highly elongated cells, is predominantly affected by cell shape rather than by the structural features of SFs. As for the mechanotaxis behaviors induced on MEG gel, design of both elasticity jump and discreteness in the elasticity boundary was found to be essential to cause cellular directional movement.
AB - To investigate the shape-dependent cell mechanics and ECM dependent control of motility, cell elasticity, structural features of the actin cytoskeleton (CSK) and motility responses were characterized on well-designed microprocessed substrates with micropatterned cell adhesive regions and microelastic gradient. Cell elasticity and atin CSK features of shape-engineered fibroblasts and vascular endothelial cells (round and spindle shaped) cultured on photolithographically microprocessed surfaces were characterized employing the cellular microindentation test and fluorescence observation of actin CSK by the combination of atomic force microscopy (AFM) and fluorescence microscopy (FM). Condition of directional cell movement towards stiffer region, so called mechanotaxis, was studied on the photolithographically-microprocessed microelastic gradient gel (MEG gel). The inter relationships between cell elasticity, the structural features of actin CSK and engineered cell shape were analyzed as compared with control cells cultured on non-processed surfaces (termed naturally extended cells). The results showed that the elasticity of regionally restricted adhesion-surface-induced shape-engineered cells, particularly highly elongated cells, is predominantly affected by cell shape rather than by the structural features of SFs. As for the mechanotaxis behaviors induced on MEG gel, design of both elasticity jump and discreteness in the elasticity boundary was found to be essential to cause cellular directional movement.
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U2 - 10.1109/MHS.2006.320327
DO - 10.1109/MHS.2006.320327
M3 - Conference contribution
AN - SCOPUS:50449086303
SN - 1424407176
SN - 9781424407170
T3 - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
BT - 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
Y2 - 5 November 2006 through 8 November 2006
ER -