Characterization of the genomic structure and expression of the mouse Apex2 gene

Yasuhito Ide, Daisuke Tsuchimoto, Yohei Tominaga, Yukihide Iwamoto, Yusaku Nakabeppu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We isolated a mouse cDNA encoding APEX2 protein and demonstrated that APEX2 binds to PCNA. The level of Apex2 mRNA was high in the thymus, bone marrow, spleen, and kidney in adult mice. Apex2 consists of six exons and is flanked on the 3′ end by Alas2 on X chromosome 63.0. Furthermore, Apex2 is flanked on the 5′ end by a novel gene with a 106-bp intergenic sequence. We disrupted Apex2 in embryonic stem cells derived from a male mouse, and a 55-kDa APEX2 protein was detected in the nuclei of Apex2+ but not Apex2-disrupted cells. Immunoelectron microscopy revealed that APEX2 is also localized in the mitochondria of Apex2+ cells. In serum-stimulated BALB/c 3T3 cells, the level of Apex2 mRNA was transiently increased and the level of APEX2 reached a maximum in the late S phase, thus indicating that APEX2 may participate in postreplicative base excision repair.

Original languageEnglish
Pages (from-to)47-57
Number of pages11
JournalGenomics
Volume81
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

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BALB 3T3 Cells
Genes
Messenger RNA
Intergenic DNA
Immunoelectron Microscopy
Proliferating Cell Nuclear Antigen
X Chromosome
Embryonic Stem Cells
S Phase
DNA Repair
Thymus Gland
Exons
Mitochondria
Proteins
Spleen
Complementary DNA
Bone Marrow
Kidney
Serum

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Characterization of the genomic structure and expression of the mouse Apex2 gene. / Ide, Yasuhito; Tsuchimoto, Daisuke; Tominaga, Yohei; Iwamoto, Yukihide; Nakabeppu, Yusaku.

In: Genomics, Vol. 81, No. 1, 01.01.2003, p. 47-57.

Research output: Contribution to journalArticle

Ide, Yasuhito ; Tsuchimoto, Daisuke ; Tominaga, Yohei ; Iwamoto, Yukihide ; Nakabeppu, Yusaku. / Characterization of the genomic structure and expression of the mouse Apex2 gene. In: Genomics. 2003 ; Vol. 81, No. 1. pp. 47-57.
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