Characterization of the porphyromonas gingivalis conjugative transposon CTnPg1: Determination of the integration site and the genes essential for conjugal transfer

Mariko Naito, Keiko Sato, Mikio Shoji, Hideharu Yukitake, Yoshitoshi Ogura, Tetsuya Hayashi, Koji Nakayama

Research output: Contribution to journalArticle

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Abstract

In our previous study, extensive genomic rearrangements were found in two strains of the Gramnegative anaerobic bacterium Porphyromonas (Por.) gingivalis, and most of these rearrangements were associated with mobile genetic elements such as insertion sequences and conjugative transposons (CTns). CTnPg1, identified in Por. gingivalis strain ATCC 33277, was the first complete CTn reported for the genus Porphyromonas. In the present study, we found that CTnPg1 can be transferred from strain ATCC 33277 to another Por. gingivalis strain, W83, at a frequency of 10 -7 to 10 -6. The excision of CTnPg1 from the chromosome in a donor cell depends on an integrase (Int; PGN_0094) encoded in CTnPg1, whereas CTnPg1 excision is independent of PGN_0084 (a DNA topoisomerase I homologue; Exc) encoded within CTnPg1 and recA (PGN_1057) on the donor chromosome. Intriguingly, however, the transfer of CTnPg1 between Por. gingivalis strains requires RecA function in the recipient. Sequencing analysis of CTnPg1- integrated sites on the chromosomes of transconjugants revealed that the consensus attachment (att) sequence is a 13 bp sequence, TTTTCNNNNAAAA. We further report that CTnPg1 is able to transfer to two other bacterial species, Bacteroides thetaiotaomicron and Prevotella oralis. In addition, CTnPg1-like CTns are located in the genomes of other oral anaerobic bacteria, Porphyromonas endodontalis, Prevotella buccae and Prevotella intermedia, with the same consensus att sequence. These results suggest that CTns in the CTnPg1 family are widely distributed among oral anaerobic Gram-negative bacteria found in humans and play important roles in horizontal gene transfer among these bacteria.

Original languageEnglish
Pages (from-to)2022-2032
Number of pages11
JournalMicrobiology
Volume157
Issue number7
DOIs
Publication statusPublished - Jul 1 2011

Fingerprint

Porphyromonas gingivalis
Essential Genes
Prevotella
Anaerobic Bacteria
Chromosomes
Consensus Sequence
Porphyromonas endodontalis
Gram-Negative Anaerobic Bacteria
Porphyromonas
Prevotella intermedia
Interspersed Repetitive Sequences
Integrases
Horizontal Gene Transfer
Type I DNA Topoisomerase
Cheek
Insertional Mutagenesis
Genome
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Characterization of the porphyromonas gingivalis conjugative transposon CTnPg1 : Determination of the integration site and the genes essential for conjugal transfer. / Naito, Mariko; Sato, Keiko; Shoji, Mikio; Yukitake, Hideharu; Ogura, Yoshitoshi; Hayashi, Tetsuya; Nakayama, Koji.

In: Microbiology, Vol. 157, No. 7, 01.07.2011, p. 2022-2032.

Research output: Contribution to journalArticle

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