Characterization of the structure and expression of mouse Itpa gene and its related sequences in the mouse genome

Mehrdad Behmanesh, Sakumi Kunihiko, Daisuke Tsuchimoto, kumiko torisu, Yoko Ohnishi-Honda, Derrick E. Rancourt, Yusaku Nakabeppu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the mouse genome, we found one processed Itpa gene-like sequence and two processed Itpa pseudogenes as well as the Itpa gene itself with introns, located on chromosome 2F3, which was isolated by a retrorecombination method. We also identified three types (A, B, C) of Itpa transcripts in mouse tissues. The processed Itpa gene-like sequence located on chromosome 2E1 has a complete open reading frame for exactly the same polypeptide as ITPA encoded by the type A transcript, with a polyadenylation signal. However, no transcribed sequence derived from the Itpa gene-like sequence was detectable in any of the mouse tissues examined, thus naming the sequence as Itpa processed pseudogene α. The type A Itpa mRNA, which was expressed in all mouse tissues examined, only encodes mouse ITPA polypeptide consisting of 198 amino acid residues with a capacity to hydrolyze dITP into dIMP. Itpa mRNA was detected in all tissues examined, and its expression is especially high in the testis, brain, and thymus. ITPA protein was mostly detected in the cytoplasm, to a lesser extent in the nuclei of neurons in the brain, and also those of hepatocytes, epithelial cells lining the bile duct, and endothelial cells lining the portal vein in the liver.

Original languageEnglish
Pages (from-to)39-51
Number of pages13
JournalDNA Research
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 1 2005

Fingerprint

Pseudogenes
Genome
Genes
Chromosomes
Messenger RNA
Peptides
Polyadenylation
Brain
Portal Vein
Bile Ducts
Introns
Thymus Gland
Open Reading Frames
Testis
Hepatocytes
Cytoplasm
Endothelial Cells
Epithelial Cells
Neurons
Amino Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Characterization of the structure and expression of mouse Itpa gene and its related sequences in the mouse genome. / Behmanesh, Mehrdad; Kunihiko, Sakumi; Tsuchimoto, Daisuke; torisu, kumiko; Ohnishi-Honda, Yoko; Rancourt, Derrick E.; Nakabeppu, Yusaku.

In: DNA Research, Vol. 12, No. 1, 01.01.2005, p. 39-51.

Research output: Contribution to journalArticle

@article{1007e16411354e4eb98b35db3b69f916,
title = "Characterization of the structure and expression of mouse Itpa gene and its related sequences in the mouse genome",
abstract = "In the mouse genome, we found one processed Itpa gene-like sequence and two processed Itpa pseudogenes as well as the Itpa gene itself with introns, located on chromosome 2F3, which was isolated by a retrorecombination method. We also identified three types (A, B, C) of Itpa transcripts in mouse tissues. The processed Itpa gene-like sequence located on chromosome 2E1 has a complete open reading frame for exactly the same polypeptide as ITPA encoded by the type A transcript, with a polyadenylation signal. However, no transcribed sequence derived from the Itpa gene-like sequence was detectable in any of the mouse tissues examined, thus naming the sequence as Itpa processed pseudogene α. The type A Itpa mRNA, which was expressed in all mouse tissues examined, only encodes mouse ITPA polypeptide consisting of 198 amino acid residues with a capacity to hydrolyze dITP into dIMP. Itpa mRNA was detected in all tissues examined, and its expression is especially high in the testis, brain, and thymus. ITPA protein was mostly detected in the cytoplasm, to a lesser extent in the nuclei of neurons in the brain, and also those of hepatocytes, epithelial cells lining the bile duct, and endothelial cells lining the portal vein in the liver.",
author = "Mehrdad Behmanesh and Sakumi Kunihiko and Daisuke Tsuchimoto and kumiko torisu and Yoko Ohnishi-Honda and Rancourt, {Derrick E.} and Yusaku Nakabeppu",
year = "2005",
month = "1",
day = "1",
doi = "10.1093/dnares/12.1.39",
language = "English",
volume = "12",
pages = "39--51",
journal = "DNA Research",
issn = "1340-2838",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Characterization of the structure and expression of mouse Itpa gene and its related sequences in the mouse genome

AU - Behmanesh, Mehrdad

AU - Kunihiko, Sakumi

AU - Tsuchimoto, Daisuke

AU - torisu, kumiko

AU - Ohnishi-Honda, Yoko

AU - Rancourt, Derrick E.

AU - Nakabeppu, Yusaku

PY - 2005/1/1

Y1 - 2005/1/1

N2 - In the mouse genome, we found one processed Itpa gene-like sequence and two processed Itpa pseudogenes as well as the Itpa gene itself with introns, located on chromosome 2F3, which was isolated by a retrorecombination method. We also identified three types (A, B, C) of Itpa transcripts in mouse tissues. The processed Itpa gene-like sequence located on chromosome 2E1 has a complete open reading frame for exactly the same polypeptide as ITPA encoded by the type A transcript, with a polyadenylation signal. However, no transcribed sequence derived from the Itpa gene-like sequence was detectable in any of the mouse tissues examined, thus naming the sequence as Itpa processed pseudogene α. The type A Itpa mRNA, which was expressed in all mouse tissues examined, only encodes mouse ITPA polypeptide consisting of 198 amino acid residues with a capacity to hydrolyze dITP into dIMP. Itpa mRNA was detected in all tissues examined, and its expression is especially high in the testis, brain, and thymus. ITPA protein was mostly detected in the cytoplasm, to a lesser extent in the nuclei of neurons in the brain, and also those of hepatocytes, epithelial cells lining the bile duct, and endothelial cells lining the portal vein in the liver.

AB - In the mouse genome, we found one processed Itpa gene-like sequence and two processed Itpa pseudogenes as well as the Itpa gene itself with introns, located on chromosome 2F3, which was isolated by a retrorecombination method. We also identified three types (A, B, C) of Itpa transcripts in mouse tissues. The processed Itpa gene-like sequence located on chromosome 2E1 has a complete open reading frame for exactly the same polypeptide as ITPA encoded by the type A transcript, with a polyadenylation signal. However, no transcribed sequence derived from the Itpa gene-like sequence was detectable in any of the mouse tissues examined, thus naming the sequence as Itpa processed pseudogene α. The type A Itpa mRNA, which was expressed in all mouse tissues examined, only encodes mouse ITPA polypeptide consisting of 198 amino acid residues with a capacity to hydrolyze dITP into dIMP. Itpa mRNA was detected in all tissues examined, and its expression is especially high in the testis, brain, and thymus. ITPA protein was mostly detected in the cytoplasm, to a lesser extent in the nuclei of neurons in the brain, and also those of hepatocytes, epithelial cells lining the bile duct, and endothelial cells lining the portal vein in the liver.

UR - http://www.scopus.com/inward/record.url?scp=18244379589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18244379589&partnerID=8YFLogxK

U2 - 10.1093/dnares/12.1.39

DO - 10.1093/dnares/12.1.39

M3 - Article

C2 - 16106751

AN - SCOPUS:18244379589

VL - 12

SP - 39

EP - 51

JO - DNA Research

JF - DNA Research

SN - 1340-2838

IS - 1

ER -