Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera

Masahiro Funada, Shinobu Yasuo, Takashi Yoshimura, Shizufumi Ebihara, Hiromi Sasagawa, Yasuo Kitagawa, Tatsuhiko Kadowaki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

L-Glutamate is a major neurotransmitter at the excitatory synapses in the vertebrate brain. It is also the excitatory neurotransmitter at neuromuscular junctions in insects, however its functions in their brains remain to be established. We identified and characterized two different subtypes (AmGluRA and AmGluRB) of metabotropic glutamate receptors (mGluRs) from an eusocial insect, honeybee. Both AmGluRA and AmGluRB form homodimers independently on disulfide bonds, and bind [3H]glutamate with KD values of 156.7 and 80.7 nM, respectively. AmGluRB is specifically expressed in the brain, while AmGluRA is expressed in the brain and other body parts, suggesting that AmGluRA is also present at the neuromuscular junctions. Both mGluRs are expressed in the mushroom bodies and the brain regions of honeybees, where motor neurons are clustered. Their expression in the brain apparently overlaps, suggesting that they may interact with each other to modulate the glutamatergic neurotransmission.

Original languageEnglish
Pages (from-to)190-194
Number of pages5
JournalNeuroscience Letters
Volume359
Issue number3
DOIs
Publication statusPublished - Apr 15 2004
Externally publishedYes

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Metabotropic Glutamate Receptors
Bees
Brain
Neuromuscular Junction
Neurotransmitter Agents
Insects
Glutamic Acid
Mushroom Bodies
Body Regions
Motor Neurons
Human Body
Synaptic Transmission
Disulfides
Synapses
Vertebrates

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera. / Funada, Masahiro; Yasuo, Shinobu; Yoshimura, Takashi; Ebihara, Shizufumi; Sasagawa, Hiromi; Kitagawa, Yasuo; Kadowaki, Tatsuhiko.

In: Neuroscience Letters, Vol. 359, No. 3, 15.04.2004, p. 190-194.

Research output: Contribution to journalArticle

Funada, Masahiro ; Yasuo, Shinobu ; Yoshimura, Takashi ; Ebihara, Shizufumi ; Sasagawa, Hiromi ; Kitagawa, Yasuo ; Kadowaki, Tatsuhiko. / Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera. In: Neuroscience Letters. 2004 ; Vol. 359, No. 3. pp. 190-194.
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