Characterization of transgenic tobacco with an increased α-linolenic acid level

Tatsurou Hamada, Hiroaki Kodama, Keizo Takeshita, Hideo Utsumi, Koh Iba

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Microsomal ω-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (α-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes. Transgenic tobacco (Nicotiana tabacum) plants with increased 18:3 contents (designated SIIn plants) were produced through the introduction of a construct with the tobacco microsomal ω-3 fatty acid desaturase gene under the control of the highly efficient promoter containing the E12Ω sequence. 18:3 contents in the SIIn plants were increased by about 40% in roots and by about 10% in leaves compared with the control plants. With regard to growth at 15°C and 25°C and the ability to tolerate chilling at 1°C and 5°C, there were no discernible differences between the SIIn and the control plants. Freezing tolerance in leaves and roots, which was assessed by electrolyte leakage, was almost the same between the SIIn and the control plants. The fluidity of plasma membrane from the SIIn plants was almost the same as that of the control plants. These results indicate that an increase in the 18:3 level in phospholipids is not directly involved in compensation for the diminishment in growth or membrane properties observed under low temperatures.

Original languageEnglish
Pages (from-to)591-598
Number of pages8
JournalPlant physiology
Volume118
Issue number2
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

alpha-Linolenic Acid
linolenic acid
Tobacco
tobacco
genetically modified organisms
Fatty Acid Desaturases
stearoyl-CoA desaturase
Phospholipids
phospholipids
Membranes
Linoleic Acid
Growth
cold tolerance
Nicotiana tabacum
Freezing
Electrolytes
electrolytes
linoleic acid
leaves
plasma membrane

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Characterization of transgenic tobacco with an increased α-linolenic acid level. / Hamada, Tatsurou; Kodama, Hiroaki; Takeshita, Keizo; Utsumi, Hideo; Iba, Koh.

In: Plant physiology, Vol. 118, No. 2, 01.01.1998, p. 591-598.

Research output: Contribution to journalArticle

Hamada, Tatsurou ; Kodama, Hiroaki ; Takeshita, Keizo ; Utsumi, Hideo ; Iba, Koh. / Characterization of transgenic tobacco with an increased α-linolenic acid level. In: Plant physiology. 1998 ; Vol. 118, No. 2. pp. 591-598.
@article{734762ebbe63422aa9ef280c3ef3fb58,
title = "Characterization of transgenic tobacco with an increased α-linolenic acid level",
abstract = "Microsomal ω-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (α-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes. Transgenic tobacco (Nicotiana tabacum) plants with increased 18:3 contents (designated SIIn plants) were produced through the introduction of a construct with the tobacco microsomal ω-3 fatty acid desaturase gene under the control of the highly efficient promoter containing the E12Ω sequence. 18:3 contents in the SIIn plants were increased by about 40{\%} in roots and by about 10{\%} in leaves compared with the control plants. With regard to growth at 15°C and 25°C and the ability to tolerate chilling at 1°C and 5°C, there were no discernible differences between the SIIn and the control plants. Freezing tolerance in leaves and roots, which was assessed by electrolyte leakage, was almost the same between the SIIn and the control plants. The fluidity of plasma membrane from the SIIn plants was almost the same as that of the control plants. These results indicate that an increase in the 18:3 level in phospholipids is not directly involved in compensation for the diminishment in growth or membrane properties observed under low temperatures.",
author = "Tatsurou Hamada and Hiroaki Kodama and Keizo Takeshita and Hideo Utsumi and Koh Iba",
year = "1998",
month = "1",
day = "1",
doi = "10.1104/pp.118.2.591",
language = "English",
volume = "118",
pages = "591--598",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "2",

}

TY - JOUR

T1 - Characterization of transgenic tobacco with an increased α-linolenic acid level

AU - Hamada, Tatsurou

AU - Kodama, Hiroaki

AU - Takeshita, Keizo

AU - Utsumi, Hideo

AU - Iba, Koh

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Microsomal ω-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (α-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes. Transgenic tobacco (Nicotiana tabacum) plants with increased 18:3 contents (designated SIIn plants) were produced through the introduction of a construct with the tobacco microsomal ω-3 fatty acid desaturase gene under the control of the highly efficient promoter containing the E12Ω sequence. 18:3 contents in the SIIn plants were increased by about 40% in roots and by about 10% in leaves compared with the control plants. With regard to growth at 15°C and 25°C and the ability to tolerate chilling at 1°C and 5°C, there were no discernible differences between the SIIn and the control plants. Freezing tolerance in leaves and roots, which was assessed by electrolyte leakage, was almost the same between the SIIn and the control plants. The fluidity of plasma membrane from the SIIn plants was almost the same as that of the control plants. These results indicate that an increase in the 18:3 level in phospholipids is not directly involved in compensation for the diminishment in growth or membrane properties observed under low temperatures.

AB - Microsomal ω-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (α-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes. Transgenic tobacco (Nicotiana tabacum) plants with increased 18:3 contents (designated SIIn plants) were produced through the introduction of a construct with the tobacco microsomal ω-3 fatty acid desaturase gene under the control of the highly efficient promoter containing the E12Ω sequence. 18:3 contents in the SIIn plants were increased by about 40% in roots and by about 10% in leaves compared with the control plants. With regard to growth at 15°C and 25°C and the ability to tolerate chilling at 1°C and 5°C, there were no discernible differences between the SIIn and the control plants. Freezing tolerance in leaves and roots, which was assessed by electrolyte leakage, was almost the same between the SIIn and the control plants. The fluidity of plasma membrane from the SIIn plants was almost the same as that of the control plants. These results indicate that an increase in the 18:3 level in phospholipids is not directly involved in compensation for the diminishment in growth or membrane properties observed under low temperatures.

UR - http://www.scopus.com/inward/record.url?scp=0001723353&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001723353&partnerID=8YFLogxK

U2 - 10.1104/pp.118.2.591

DO - 10.1104/pp.118.2.591

M3 - Article

AN - SCOPUS:0001723353

VL - 118

SP - 591

EP - 598

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

ER -