Characterization of transient expression system for retroviral vector production

Akitsu Hotta, Yoshikazu Saito, Kenji Kyogoku, Yoshinori Kawabe, Ken ichi Nishijima, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The production of retroviral vectors using a transient expression system has been improved to obtain a high-titer virus preparation that is difficult to produce using packaging cell lines due to the cytotoxic or cytostatic effect of transgenes. Here, we used one such production method, the so-called Q-vector system, and examined its potential for virus production. The Q-vector system could produce a similar level of viral vectors compared with the packaging cell system but the production seemed to depend on the size and nature of transgenes. In the process of investigation of the quantitative difference in viral components between the transient expression system and the packaging cell system, we found that the Q-vector system could express higher amounts of viral RNA and proteins compared with the packaging cell system. However, this did not lead to a higher virus titer compared with that produced by the packaging cell system. This suggests that retroviral RNA transcribed from the plasmid in the transient system seemed to be used mainly for translation and only some of the RNA molecules were packaged in viral particles.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalJournal of Bioscience and Bioengineering
Volume101
Issue number4
DOIs
Publication statusPublished - Apr 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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