Characterization of tudor-sn-containing granules in the silkworm, bombyx mori

Li Zhu, Tsuneyuki Tatsuke, Hiroaki Mon, Zhiqing Li, Jian Xu, Man Lee, Takahiro Kusakabe

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Tudor-sn protein, which contains four staphylococcal nuclease domains and a Tudor domain, is a ubiquitous protein found in almost all organisms. It has been reported that Tudor-sn in mammals participates in various cellular pathways involved in gene regulation, cell growth, and development. In insects, we have previously identified a Tudor-sn ortholog in the silkworm, Bombyx mori, and detected its interactions between with Argonaute proteins. The role of Tudor-sn in silkworm, however, still remains largely unknown. In this study, we demonstrated that silkworm Tudor-sn is a stress granule (SG) protein, and determined its interactions with other SG proteins using Bimolecular Fluorescence Complementation assay and Insect Two-Hybrid method. Depletions of Argonaute proteins and SG-marker protein Tia1 by RNAi impaired the involvement of Tudor-sn in the SG formation. Protein domain deletion analysis of Tudor-sn demonstrated that SN2 is the key domain required for the aggregation of Tudor-sn in SGs.

Original languageEnglish
Pages (from-to)664-674
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume43
Issue number8
DOIs
Publication statusPublished - Aug 1 2013

Fingerprint

Bombyx
Bombyx mori
silkworms
granules
Argonaute Proteins
Heat-Shock Proteins
Insects
Proteins
proteins
Micrococcal Nuclease
Two-Hybrid System Techniques
RNA Interference
Growth and Development
Mammals
micrococcal nuclease
Fluorescence
Cell growth
insects
protein depletion
Gene expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Characterization of tudor-sn-containing granules in the silkworm, bombyx mori. / Zhu, Li; Tatsuke, Tsuneyuki; Mon, Hiroaki; Li, Zhiqing; Xu, Jian; Lee, Man; Kusakabe, Takahiro.

In: Insect Biochemistry and Molecular Biology, Vol. 43, No. 8, 01.08.2013, p. 664-674.

Research output: Contribution to journalArticle

Zhu, Li ; Tatsuke, Tsuneyuki ; Mon, Hiroaki ; Li, Zhiqing ; Xu, Jian ; Lee, Man ; Kusakabe, Takahiro. / Characterization of tudor-sn-containing granules in the silkworm, bombyx mori. In: Insect Biochemistry and Molecular Biology. 2013 ; Vol. 43, No. 8. pp. 664-674.
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