Characterization of two Cucumber mosaic virus isolated from Solanum mammosum and Nicotiana affinis

Marcela A. Tamashiro, Minoru Takeshita, Naruto Furuya, Yoichi Takanami

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two Cucumber mosaic virus (CMV) isolates, CMV-NA from Nicotiana affinis, and CMV-SM from Solanum mammosum (nipple fruit plant) inducing mild and severe systemic mosaic symptoms, respectively, on tobacco (Nicotiana tabacum cv. Xanthi-nc), were characterized on the basis of their nucleotide and amino acid sequences of RNA3, serology and host ranges. The complete nucleotide sequencing of RNA3s of CMV-NA and CMV-SM has been done. Multiple alignment analysis of the nucleotide sequences of RNA3 of the 5′noncoding region and amino acid sequences of the CP and MP revealed that, CMV-NA and CMV-SM belong to subgroup I. CMV-NA induced systemic infection on Cryptotaenia japonica and caused severe leaf malformation, but CMV-SM could not infect this plant. N. occidentalis inoculated mechanically with CMV-SM developed mild mosaic symptoms, while CMV-NA was unable to induce systemic infection on this plant. Thus, C. japonica and N. occidentalis are strain specific hosts for CMV-NA and CMV-SM, respectively. Raphanus sativus cv. Akidumari and Phaseolus vulgaris demonstrated to be non-host plants of CMV-NA and CMV-SM. This is the first report of a CMV strain infecting N. affinis in the field.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume49
Issue number2
Publication statusPublished - Oct 1 2004

Fingerprint

Solanum mammosum
Cucumovirus
Nicotiana alata
Solanum
Cucumber mosaic virus
Tobacco
vinyl polysiloxane
signs and symptoms (plants)
Cryptotaenia
Amino Acid Sequence
amino acid sequences
Nucleotides
nucleotides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Characterization of two Cucumber mosaic virus isolated from Solanum mammosum and Nicotiana affinis. / Tamashiro, Marcela A.; Takeshita, Minoru; Furuya, Naruto; Takanami, Yoichi.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 49, No. 2, 01.10.2004, p. 243-252.

Research output: Contribution to journalArticle

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