Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast

Shotaro Suzuki, Tomohiko Matsuzawa, Yayoi Nukigi, Kaoru Takegawa, Naotaka Tanaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Schizosaccharomyces species are currently the only known organisms with two types of genes encoding UDP-glucose/-galactose 4-epimerase, uge1+ and gal10+. A strain deleted for uge1+ exhibited a severe galactosylation defect and a decrease in activity and in UDP-galactose content when grown in glucose-rich medium (2% glucose), indicating that Uge1p is a major UDP-glucose/-galactose 4-epimerase under these growth conditions. In contrast, gal10+ was efficiently expressed and involved in galactosylation of cell-surface proteins in low-glucose medium (0.1% glucose and 2% glycerol), but not in galactose-containing medium. In a uge1Δgal10Δ strain, the galactosylation defect was suppressed and UDP-galactose content restored to wild-type levels in galactose-containing medium. Disruption of gal7+, encoding galactose-1-phosphate uridylyltransferase, in the uge1Δ gal10Δ strain reversed suppression of the galactosylation defect and reduced levels of UDP-galactose, indicating that galactose is transported from the medium to the cytosol and is converted into UDP-galactose via galactose 1-phosphate by Gal7p in Sch. pombe.

Original languageEnglish
Pages (from-to)708-718
Number of pages11
JournalMicrobiology
Volume156
Issue number3
DOIs
Publication statusPublished - Mar 12 2010

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UDPglucose 4-Epimerase
Uridine Diphosphate Galactose
Schizosaccharomyces
Galactose
Glucose
Cytosol
Glycerol
Membrane Proteins
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast. / Suzuki, Shotaro; Matsuzawa, Tomohiko; Nukigi, Yayoi; Takegawa, Kaoru; Tanaka, Naotaka.

In: Microbiology, Vol. 156, No. 3, 12.03.2010, p. 708-718.

Research output: Contribution to journalArticle

Suzuki, Shotaro ; Matsuzawa, Tomohiko ; Nukigi, Yayoi ; Takegawa, Kaoru ; Tanaka, Naotaka. / Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast. In: Microbiology. 2010 ; Vol. 156, No. 3. pp. 708-718.
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