Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe

Tomoko Iwaki, Fumi Osawa, Masayuki Onishi, Takako Koga, Yasuko Fujita, Akira Hosomi, Naotaka Tanaka, Yasuhisa Fukui, Kaoru Takegawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

From the fission yeast Schizosaccharomyces pombe we have identified and deleted vps33, a gene encoding a homologue of VPS33, which is required for vacuolar biogenesis in S. cerevisiae cells. When the vps33+ gene is disrupted, Sz. pombe strains are temperature-sensitive for growth and contain numerous small vesicular structures stained with FM4-64 in the cells. Deletion of the Sz. pombe vps33+ gene results in pleiotropic phenotypes consistent with the absence of normal vacuoles, including missorting of vacuolar carboxypeptidase Y, various ion- and drug-sensitivities, and sporulation defects. These results are consistent with Vps33p being necessary for the morphogenesis of vacuoles and subsequent expression of vacuolar functions in Sz. pombe cells.

Original languageEnglish
Pages (from-to)845-855
Number of pages11
JournalYeast
Volume20
Issue number10
DOIs
Publication statusPublished - Jul 1 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

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    Iwaki, T., Osawa, F., Onishi, M., Koga, T., Fujita, Y., Hosomi, A., Tanaka, N., Fukui, Y., & Takegawa, K. (2003). Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe. Yeast, 20(10), 845-855. https://doi.org/10.1002/yea.1011